Determining the Different Mechanisms Used by Pseudomonas Species to Cope With Minimal Inhibitory Concentrations of Zinc via Comparative Transcriptomic Analyses.
Determining the Different Mechanisms Used by Pseudomonas Species to Cope With Minimal Inhibitory Concentrations of Zinc via Comparative Transcriptomic Analyses.
复制标题
通过比较转录组分析确定假单胞菌用于应对锌的最低抑制浓度的不同机制
DOI：
10.3389/fmicb.2020.573857
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Lei L;Chen J;Liao W;Liu P
假单胞菌属是环境中鉴定的最多样化的细菌属之一。基因组序列分析表明，该属可分为3个谱系和10个类群。每个群体都可以采用不同的机制在锌耗尽或高锌条件下茁壮成长，这两种环境在其环境传播过程中经常遇到。使用RNA-seq和超高效液相色谱-串联质谱分析比较了三种主要假单胞菌菌株（铜绿假单胞菌PAO 1、恶臭假单胞菌KT 2440和荧光假单胞菌ATCC 13525 T）对最小抑制浓度锌的反应。结果表明，这三个菌株在转录水平上只有最小的相似性。只有四个基因负责锌外流通常上调。铜绿假单胞菌PAO 1特异性下调参与铁载体合成的操纵子和编码核糖体蛋白的基因，而上调与抗生素外排和细胞包膜生物合成相关的基因。恶臭假单胞菌KT 2440中的膜转运蛋白整体下调，表明细胞通透性的变化。与铜绿假单胞菌PAO 1和恶臭假单胞菌KT 2440相比，荧光假单胞菌ATCC 13525 T中最显著的转录变异是VI型分泌系统的显著下调。代谢产物定量分析表明，三株菌均检测到低浓度的参与中心碳代谢和氨基酸合成的代谢产物。综上所述，三株菌在高锌条件下的细胞反应存在较大差异。虽然类似的金属外排系统上调，三种菌株采用不同的途径，以减少锌入侵。此外，锌处理会增加铜绿假单胞菌从定植区域清除的难度，降低荧光假单胞菌在微生物群中的竞争力。
Pseudomonas is one of the most diverse bacterial genera identified in the environment. Genome sequence analysis has indicated that this genus can be clustered into three lineages and ten groups. Each group can adopt different mechanisms to thrive under zinc-depleted or high-zinc conditions, two environments that are frequently encountered during their environmental propagation. The response of three prominent Pseudomonas strains (Pseudomonas aeruginosa PAO1, Pseudomonas putida KT2440, and Pseudomonas fluorescens ATCC 13525T) to minimal inhibitory concentrations of zinc were compared using RNA-seq and ultra-performance liquid chromatography–tandem mass spectrometry analysis. Results demonstrated that the three strains shared only minimal similarity at the transcriptional level. Only four genes responsible for zinc efflux were commonly upregulated. P. aeruginosa PAO1 specifically downregulated the operons involved in siderophore synthesis and the genes that encode ribosomal protein, while upregulated the genes associated with antibiotic efflux and cell envelope biosynthesis. The membrane transporters in P. putida KT2440 were globally downregulated, indicating changes in cell permeability. Compared with P. aeruginosa PAO1 and P. putida KT2440, the most remarkable transcriptional variation in P. fluorescens ATCC 13525T is the significant downregulation of the type VI secretion system. Metabolite quantitative analysis showed that low concentrations of the metabolites involved in central carbon metabolism and amino acid synthesis were detected in the three strains. In summary, the cellular responses of the three strains under high-zinc condition is quite divergent. Although similar metal efflux systems were upregulated, the three strains employed different pathways to reduce zinc intrusion. In addition, zinc treatment can increase the difficulties of scavenging P. aeruginosa from its colonization area, and reduce the competitiveness of P. fluorescens in microbiota.
登录
查看更多内容
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.7
作者：
Alteri CJ;Mobley HLT
通讯作者：
Mobley HLT
DOI：
10.1007/978-1-4939-0473-0_24
发表时间：
2014-01-01
期刊：
PSEUDOMONAS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Hoegy, Francoise;Mislin, Gaetan L. A.;Schalk, Isabelle J.
通讯作者：
Schalk, Isabelle J.
假单胞菌 T6SS 效应子招募含有 PQS 的外膜囊泡来获取铁
影响因子：
16.6
作者：
Lin J;Zhang W;Cheng J;Yang X;Zhu K;Wang Y;Wei G;Qian PY;Luo ZQ;Shen X
通讯作者：
Shen X
影响因子：
6.1
作者：
Gomez-Sagasti, Maria T.;Becerril, Jose M.;Garbisu, Carlos
通讯作者：
Garbisu, Carlos