Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats.
Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats.
复制标题
DOI：
10.1016/j.resp.2010.11.007
复制
发表时间：
2011-02-15
影响因子：
2.3
通讯作者：
Bavis, Ryan W.
中科院分区：
文献类型：
--
作者：
Dmitrieff, Elizabeth F.;Wilson, Julia T.;Dunmire, Kyle B.;Bavis, Ryan W.
关键词：
慢性高氧暴露改变大鼠颈动脉体的生后发育和神经支配。我们推测这种可塑性与神经营养因子或相关蛋白表达的变化有关。从出生前24-36 h，将大鼠饲养在60% O2中，直至3日龄（P3）进行研究。在P3颈动脉体中，脑源性神经营养因子（BDNF）的蛋白水平显著降低（-70%），而其受体酪氨酸激酶B和胶质细胞系源性神经营养因子（GDNF）的蛋白水平没有变化。颈动脉体中GDNF受体Ret（-34%）和神经肽Vgf（-67%）的转录水平下调，Cbln 1（+205%）上调，Fgf 2不变;这些基因的蛋白质水平没有定量。免疫组织化学分析显示，Vgf和Cbln 1蛋白表达的颈动脉体球细胞内。这些数据表明，脑源性神经营养因子，也许其他神经营养因子，有助于颈动脉体功能异常围产期高氧。
Chronic exposure to hyperoxia alters the postnatal development and innervation of the rat carotid body. We hypothesized that this plasticity is related to changes in the expression of neurotrophic factors or related proteins. Rats were reared in 60% O2 from 24–36 h prior to birth until studied at 3 d of age (P3). Protein levels for brain-derived neurotrophic factor (BDNF) were significantly reduced (−70%) in the P3 carotid body, while protein levels for its receptor, tyrosine kinase B, and for glial cell line derived neurotrophic factor (GDNF) were unchanged. Transcript levels in the carotid body were downregulated for the GDNF receptor Ret (−34%) and the neuropeptide Vgf (−67%), upregulated for Cbln1 (+205%), and unchanged for Fgf2; protein levels were not quantified for these genes. Immunohistochemical analysis revealed that Vgf and Cbln1 proteins are expressed within the carotid body glomus cells. These data suggest that BDNF, and perhaps other neurotrophic factors, contribute to abnormal carotid body function following perinatal hyperoxia.
登录
查看更多内容
DOI：
10.1523/jneurosci.3145-08.2008
发表时间：
2008-09-24
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Bozdagi O;Rich E;Tronel S;Sadahiro M;Patterson K;Shapiro ML;Alberini CM;Huntley GW;Salton SR
通讯作者：
Salton SR
影响因子：
5.3
作者：
Balkowiec, A;Katz, DM
通讯作者：
Katz, DM
影响因子：
3.3
作者：
Bavis, Ryan W.;Young, Kristen M.;Rampersad, Donna A.
通讯作者：
Rampersad, Donna A.
影响因子：
3.3
作者：
Donnelly, DF;Kim, I;Carroll, JL
通讯作者：
Carroll, JL
影响因子：
2.3
作者：
Katz, DM
通讯作者：
Katz, DM