The Candida albicans histidine kinase Chk1p: signaling and cell wall mannan.
The Candida albicans histidine kinase Chk1p: signaling and cell wall mannan.
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DOI：
10.1016/j.fgb.2009.06.008
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发表时间：
2009-10
影响因子：
3
通讯作者：
Calderone, Richard
中科院分区：
文献类型：
--
作者：
Li, Dongmei;Williams, David;Lowman, Douglas;Monteiro, Mario A.;Tan, Xuan;Kruppa, Michael;Fonzi, William;Roman, Elvira;Pla, Jesus;Calderone, Richard
一些已发表的与白色念珠菌CHK1组氨酸激酶相关的功能类似于MAPK Cek1p及其同源受体Sho1p （SSU81）。为了进一步探讨这一点，我们比较了缺乏上述蛋白质的突变体，并构建了双sho1/chk1Δ零突变体来确定这些蛋白质之间的关系。我们观察到，与单个chk1Δ或sho1Δ突变体相比，双突变体对刚果红（CR）、白钙（CW）的敏感性以及细胞的结块略有增加。然而，通过Sho1p发生的Cek1p磷酸化，在Wt细胞中存在或不存在CR的log期生长过程中，不需要Chk1p。这些数据表明Chk1p和Sho1p是平行但独立的信号通路的组成部分。此外，采用GPC/MS和NMR对菌株的甘露聚糖进行了分析。与Wt和含有高、中、低Mw甘露聚糖的CHK1基因重组菌株CHK23相比，我们发现菌株CHK21 （chk1Δ null）、cek1Δ null和双突变株的甘露聚糖仅含有低Mw甘露聚糖。sho1Δ零突变体仅表现出减少的中间型甘露聚糖。Alcian blue binding在cek1Δ， chk1Δ和双sho1/chk1Δ零突变体中缺乏高和中等分子量甘露聚糖，而在sho1Δ零突变体中只有部分损失中等分子量甘露聚糖。我们得出结论，Chk1p HK是与Sho1p-Cek1p途径功能相似但平行的一部分，该途径赋予对细胞壁抑制剂CR和CW的抗性。然而，甘露聚糖生物合成中Chk1p和Cek1p的功能关系仅部分需要Sho1p。
Several published functions associated with the CHK1 histidine kinase of Candida albicans resemble those of the MAPK Cek1p and its cognate receptor Sho1p (SSU81). To explore this further, we have compared mutants lacking the proteins mentioned above and have constructed a double sho1/chk1Δ null mutant to determine relationships among these proteins. We observed that the sensitivity to Congo red (CR), calcofluor white (CW), as well as clumping of cells, was slightly increased in the double mutant compared to the single chk1Δ or sho1Δ mutants. However, Cek1p phosphorylation via Sho1p, which occurs during log phase growth in the presence or absence of CR in Wt cells, does not require Chk1p. These data suggest that Chk1p and Sho1p are components of parallel but independent signal pathways. In addition, bulk mannan of strains was analyzed by GPC/MS and NMR. Compared to Wt and a CHK1 gene-reconstituted strain (CHK23) that contained, high, intermediate and low Mw mannan species, we found that the mannan of strains CHK21 (chk1Δ null), the cek1Δ null, and the double mutant consisted only of low Mw mannan. The sho1Δ null mutant only demonstrated a reduced intermediate type of mannan. Alcian blue binding was lower in cek1Δ, chk1Δ, and the double sho1/chk1Δ null mutant lacking high and intermediate Mw mannan than in the sho1Δ null which had a partial loss of intermediate Mw mannan only. We conclude that the Chk1p HK is part of a functionally similar but parallel pathway to the Sho1p-Cek1p pathway that confers resistance to the cell wall inhibitors CR and CW. However, a functional relationship in mannan biosynthesis of Chk1p and Cek1p exists that only partially requires Sho1p.
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影响因子：
3.2
作者：
KOCOUREK, J;BALLOU, CE
通讯作者：
BALLOU, CE
影响因子：
3.1
作者：
Calera, JA;Zhao, XJ;Calderone, R
通讯作者：
Calderone, R
影响因子：
2.8
作者：
Arana, DM;Nombela, C;Pla, J
通讯作者：
Pla, J
影响因子：
--
作者：
Chauhan, N;Inglis, D;Calderone, R
通讯作者：
Calderone, R
影响因子：
--
作者：
Kruppa, M;Krom, BP;Calderone, RA
通讯作者：
Calderone, RA