Molecular analysis of fungal populations in patients with oral candidiasis using internal transcribed spacer region.
Molecular analysis of fungal populations in patients with oral candidiasis using internal transcribed spacer region.
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DOI：
10.1371/journal.pone.0101156
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nakamura S
中科院分区：
文献类型：
--
作者：
Ieda S;Moriyama M;Takeshita T;Maehara T;Imabayashi Y;Shinozaki S;Tanaka A;Hayashida JN;Furukawa S;Ohta M;Yamashita Y;Nakamura S
口腔念珠菌病与口腔真菌植物群的变化密切相关，主要由白色念珠菌引起。真菌培养的传统方法是耗时的，并不总是结论性的。然而，真菌rRNA的内部转录间隔区（ITS）区域的分子遗传学分析是快速的，可重复的和简单的执行。在这项研究中，我们研究了真菌植物群在口腔念珠菌病患者和抗真菌治疗后的植物群的变化，使用长度异质性聚合链反应（LH-PCR）分析ITS区。52例伪膜性口腔念珠菌病（POC）患者和30名健康对照者被纳入本研究。采用LH-PCR方法检测口腔冲洗液中真菌的种类多样性。通过实时PCR定量真菌群体，并通过核苷酸测序确认先前未识别的信号。还研究了口腔真菌植物群与治疗抵抗因素之间的关系。POC患者表现出显着更多的真菌种类和更大的真菌密度比对照组。新鉴定真菌16种。两组的真菌种群均以C. albicans，但C. dubliniensis在POC患者中显著高于对照组。处理后土壤真菌的多样性和密度明显降低。此外，真菌多样性和C。dubliniensis与治疗时间呈正相关。这些结果表明C. dubliniensis和高度的真菌植物群多样性可能参与了口腔念珠菌病的发病。因此，我们得出结论，LH-PCR是一种有用的技术，用于诊断和评估口腔念珠菌感染的严重程度。
Oral candidiasis is closely associated with changes in the oral fungal flora and is caused primarily by Candida albicans. Conventional methods of fungal culture are time-consuming and not always conclusive. However, molecular genetic analysis of internal transcribed spacer (ITS) regions of fungal rRNA is rapid, reproducible and simple to perform. In this study we examined the fungal flora in patients with oral candidiasis and investigated changes in the flora after antifungal treatment using length heterogeneity-polymerization chain reaction (LH-PCR) analysis of ITS regions. Fifty-two patients with pseudomembranous oral candidiasis (POC) and 30 healthy controls were included in the study. Fungal DNA from oral rinse was examined for fungal species diversity by LH-PCR. Fungal populations were quantified by real-time PCR and previously-unidentified signals were confirmed by nucleotide sequencing. Relationships between the oral fungal flora and treatment-resistant factors were also examined. POC patients showed significantly more fungal species and a greater density of fungi than control individuals. Sixteen fungi were newly identified. The fungal populations from both groups were composed predominantly of C. albicans, though the ratio of C. dubliniensis was significantly higher in POC patients than in controls. The diversity and density of fungi were significantly reduced after treatment. Furthermore, fungal diversity and the proportion of C. dubliniensis were positively correlated with treatment duration. These results suggest that C. dubliniensis and high fungal flora diversity might be involved in the pathogenesis of oral candidiasis. We therefore conclude that LH-PCR is a useful technique for diagnosing and assessing the severity of oral candidal infection.
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影响因子：
5.9
作者：
Ostrosky-Zeichner, Luis
通讯作者：
Ostrosky-Zeichner, Luis
影响因子：
5.8
作者：
Pasqualotto, Alessandro C.
通讯作者：
Pasqualotto, Alessandro C.
影响因子：
14.9
作者：
Liu, ZJ
通讯作者：
Liu, ZJ
影响因子：
3.6
作者：
Sharifzadeh, A.;Khosravi, A. R.;Tamami, I. Ashrafi
通讯作者：
Tamami, I. Ashrafi
影响因子：
--
作者：
Takeshita, T.;Nakano, Y.;Yamashita, Y.
通讯作者：
Yamashita, Y.