Diminished adhesion and activation of platelets and neutrophils with CD47 functionalized blood contacting surfaces.
Diminished adhesion and activation of platelets and neutrophils with CD47 functionalized blood contacting surfaces.
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与CD47功能化血接触表面的血小板和中性粒细胞的粘附和激活减少。
DOI：
10.1016/j.biomaterials.2012.04.051
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发表时间：
2012-08
期刊：
影响因子：
14
通讯作者：
Stachelek, Stanley J.
中科院分区：
文献类型：
--
作者：
Finley, Matthew J.;Rauova, Lubica;Alferiev, Ivan S.;Weisel, John W.;Levy, Robert J.;Stachelek, Stanley J.
CD 47是一种广泛表达的跨膜蛋白，通过信号调节蛋白α 1（SIRPα1）介导的信号传导机制，作为“自我修复”的生物标志物发挥作用。我们以前表明，炎症细胞附着到聚合物表面的生物素化的重组CD 47（CD 47 B）的附着抑制。我们在此测试的假设，CD 47修饰的血液导管可以减少血小板和中性粒细胞活化在临床相关条件下。我们将多聚赖氨酸标签附加到重组CD 47（CD 47 L）的C-末端，允许共价连接到聚合物。SIRPα1在分离的血小板中表达。然后，我们比较了CD 47 B和CD 47 L官能化的聚氯乙烯（PVC）表面和未改性的对照PVC表面之间的生物相容性。通过扫描电子显微镜对血细胞粘附于CD 47 B和CD 47 L表面的定量和定性分析显示，与对照相比，粘附于CD 47修饰表面的血小板显著减少。流式细胞术分析显示，暴露于CD 47修饰的PVC的中性粒细胞（CD 62 L）和血小板（CD 62 P）的活化标志物与新鲜采集的对照血液相当，而在未修饰的PVC管中显著升高。此外，环氧乙烷气体灭菌不会抑制CD 47修饰的功效。总之，CD 47修饰的PVC抑制血小板和中性粒细胞的粘附和活化。
CD47 is a ubiquitously expressed transmembrane protein that, through signaling mechanisms mediated by signal regulatory protein alpha (SIRPα1), functions as a biological marker of ‘self-recognition’. We showed previously that inflammatory cell attachment to polymeric surfaces is inhibited by the attachment of biotinylated recombinant CD47 (CD47B). We test herein the hypothesis that CD47-modified blood conduits can reduce platelet and neutrophil activation under clinically relevant conditions. We appended a poly-lysine tag to the C-terminus of recombinant CD47 (CD47L) allowing for covalent linkage to the polymer. SIRPα1 expression was confirmed in isolated platelets. We then compared biocompatibility between CD47B and CD47L functionalized polyvinyl chloride (PVC) surfaces and unmodified control PVC surfaces. Quantitative and Qualitative analysis of blood cell attachment to CD47B and CD47L surfaces, via scanning electron microscopy, showed strikingly fewer platelets attached to CD47 modified surfaces compared to control. Flow cytometry analysis showed that activation markers for neutrophils (CD62L) and platelets (CD62P) exposed to CD47 modified PVC were equivalent to freshly acquired control blood, while significantly elevated in the unmodified PVC tubing. In addition, ethylene oxide gas sterilization did not inhibit the efficacy of the CD47 modification. In conclusion, CD47 modified PVC inhibits both the adhesion and activation of platelets and neutrophils.
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影响因子：
4.6
作者：
Edmunds, LH
通讯作者：
Edmunds, LH
影响因子：
1.5
作者：
Harig, F.;Meier, C.;Cesnjevar, R.
通讯作者：
Cesnjevar, R.
影响因子：
20.3
作者：
Olsson, M;Bruhns, P;Oldenborg, PA
通讯作者：
Oldenborg, PA
影响因子：
4.9
作者：
Maitz, Manfred F.;Sperling, Claudia;Werner, Carsten
通讯作者：
Werner, Carsten
DOI：
10.1016/s0889-8537(03)00039-7
发表时间：
2003-09-01
期刊：
Anesthesiology clinics of North America
影响因子：
--
作者：
Pintar, Tatjana;Collard, Charles D
通讯作者：
Collard, Charles D