Bradykinin inhibits a potassium M‐like current in rat pheochromocytoma PC12 cells
Bradykinin inhibits a potassium M‐like current in rat pheochromocytoma PC12 cells
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缓激肽抑制大鼠嗜铬细胞瘤 PC12 细胞中的钾 M 样电流
DOI：
10.1016/0014-5793(89)81057-9
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
P. Adams
中科院分区：
文献类型：
--
作者：
A. Villarroel;N. Marrion;H. López;P. Adams
采用全细胞电压钳技术研究了缓激肽（BK）对融合性嗜铬细胞瘤PC 12细胞离子电流的作用，并利用Fura-2研究了BK对细胞内钙离子的影响。BK诱导与细胞内钙增加相关的外向电流的发展，随后抑制M样电流。该外向电流被（+）-筒箭毒碱阻断，当钙螯合剂BAPTA或高浓度的肌醇1，4，5-三磷酸被引入细胞中时被阻止，而M样电流及其被BK抑制则不受影响。蛋白激酶激活剂佛波醇12，13二丁酸酯部分降低M-电流。M-电流密度没有实质性的变化后，长期治疗神经生长因子。
We studied the action of bradykinin (BK) on ionic currents in fused pheochromocytoma PC12 cells under voltage-clamp in whole-cell mode, and on intracellular calcium using fura-2. BK induced the development of an outward current associated with an increase in intracellular calcium, followed by inhibition of an M-like current. The outward current was blocked by (+)-tubocurarine, and prevented when the calcium chelator BAPTA or high concentrations of inositol 1,4,5-triphosphate were introduced into the cell, whereas the M-like current and its inhibition by BK remained unaffected. The protein kinase activator phorbol 12,13 dibutyrate partially reduced the M-current. M-current density did not substantially change after prolonged treatment with nerve growth factor.
DOI：
10.1073/pnas.85.3.924
发表时间：
1988-02-01
影响因子：
11.1
作者：
MANDEL, G;COOPERMAN, SS;BREHM, P
通讯作者：
BREHM, P
DOI：
10.1073/pnas.86.8.2943
发表时间：
1989-04-01
影响因子：
11.1
作者：
BOSMA, MM;HILLE, B
通讯作者：
HILLE, B