Validation of Heterologous Radioimmunoassays (RIA) for Growth Hormone (GH) and Insulin-Like Growth Factor (IGF)-I in Phocid, Otariid, and Cetacean Species
Validation of Heterologous Radioimmunoassays (RIA) for Growth Hormone (GH) and Insulin-Like Growth Factor (IGF)-I in Phocid, Otariid, and Cetacean Species
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对磷类、Otariid 和鲸类物种中生长激素 (GH) 和胰岛素样生长因子 (IGF)-I 的异源放射免疫测定 (RIA) 进行验证
DOI：
10.1578/am.35.1.2009.19
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发表时间：
2009
期刊：
影响因子：
1.2
通讯作者：
S. Zinn
中科院分区：
文献类型：
--
作者：
J. P. Richmond;S. Zinn
生长激素（GH）是一种同源异型激素，其协调多种生理过程以将营养物质分配给瘦组织增生和产奶，并抑制脂肪组织对营养物质的利用，特别是在能量缺乏期间。由于生长激素与年龄相关的下降，生长激素与总体生长速度和脂质增加呈负相关，但与蛋白质增加呈正相关。循环GH直接或间接影响外周组织，主要通过胰岛素样生长因子（IGF）-I介导。与GH类似，年龄和营养影响IGF-I的分泌。一般来说，IGF-I与生长速度和蛋白质的增加呈正相关。由于纯化鳍足类动物或鲸类动物生长激素和IGF-I是不可用的，异源测定技术必须采用定量这些激素的浓度。异源测定法可靠性的关键是正确执行验证，以证明测定法对每种新感兴趣种属的准确度和精密度。在这项研究中，我们开发和验证了异源放射免疫分析七种海洋哺乳动物使用猪和人的抗血清GH和IGF-I的定量，分别。测定了各试验和种属的灵敏度、质量回收率、试验精密度、平行性和稀释线性。两种测定均表现出良好的平行性和线性。在标准曲线的整个范围内定量适当浓度的激素。重要的是，使用这些测定，我们已经证明，血清中GH和IGF-I的浓度随年龄和营养摄入量的变化，在这些物种。验证这些测定允许代谢激素的评价，可以提供不同的海洋哺乳动物物种的差异生长和营养分配的生理调节独特的观点。
Growth hormone (GH) is a homeorhetic hormone that coordinates diverse physiological processes to partition nutrients toward lean tissue accretion and milk production, and to inhibit utilization of nutrients by adipose tissue, especially during periods of energy deficiency. Due to age-related declines in GH, GH is negatively correlated with overall growth rate and lipid accretion but positively associated with protein accretion. Circulating GH influences peripheral tissues directly or indirectly and is primarily mediated via an insulin-like growth factor (IGF)-I. Similar to GH, age and nutrition influence IGF-I secretion. In general, IGF-I is positively associated with growth rate and accretion of protein. Since purified pinniped or cetacean GH and IGF-I are not available, heterologous assay techniques must be employed to quantify concentrations of these hormones. Critical to the reliability of heterologous assays are the proper execution of validations to demonstrate the accuracy and precision of the assay for each new species of interest. In this study, we developed and validated heterologous radioimmunoassays for seven marine mammal species using porcine and human antisera for GH and IGF-I quantification, respectively. Sensitivity, recovery of mass, assay precision, parallelism, and dilution linearity were determined for each assay and species. Both assays exhibited excellent parallelism and linearity. Appropriate concentrations of hormone were quantified throughout the range of the standard curve. Importantly, using these assays, we have demonstrated that serum concentrations of GH and IGF-I change with age and nutrient intake in these species. Validation of these assays allows evaluation of metabolic hormones that may provide unique perspectives on physiological regulation of differential growth and nutrient allocation for diverse marine mammal species.
影响因子：
20.3
作者：
John I. Jones;D. Clemmons
通讯作者：
John I. Jones;D. Clemmons
犬饥饿中的胰岛素样生长因子 I 和生长激素。
DOI：
10.1530/acta.0.1080161
发表时间：
1985
期刊：
Acta endocrinologica
影响因子：
--
作者：
Eigenmann,JE;deBruijne,JJ;Froesch,ER
通讯作者：
Froesch,ER
在自然适应的物种北象海豹（Mirounga angustirostris）中，GH 和生长素释放肽会随着禁食而增加。
DOI：
10.1677/joe.0.1780533
发表时间：
2003
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Ortiz,RM;Noren,DP;Ortiz,CL;Talamantes,F
通讯作者：
Talamantes,F