Identification and charactering of APX genes provide new insights in abiotic stresses response in Brassica napus.
Identification and charactering of APX genes provide new insights in abiotic stresses response in Brassica napus.
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作者：
Pan J;Zhang L;Chen M;Ruan Y;Li P;Guo Z;Liu B;Ruan Y;Xiao M;Huang Y
抗坏血酸过氧化物酶（Ascorbate peroxidase， APX）在植物细胞中清除H2O2和平衡ROS含量具有重要作用，对生命的生长发育和抵抗外界胁迫具有重要意义。然而，到目前为止，对甘蓝型油菜（B. napus）的apx还没有系统的表征。本研究共鉴定出26个BnaAPX基因，分布在13条染色体上，分为5个系统发育分支。基因结构分析表明，它们的外显子数量差异很大，而BnaAPXs蛋白在相同的系统发育分支中含有更多相似的基序。对26个BnaAPX基因表达谱的qRT-PCR分析显示，在高盐、缺水和高温胁迫下，细胞质BnaAPX 3个推定基因BnaAPX1、BnaAPX2、BnaAPX9和2个推定微体基因BnaAPX18和BnaAPX25快速而强劲地表达上调。此外，上述三种非生物胁迫均导致APX活性显著升高。为进一步研究油菜APX基因家族的功能提供了基础和全面的信息。
Ascorbate peroxidase (APX) plays an important role in scavenging H2O2 and balancing ROS content in plant cells, which is of great significance for the growth and development of life and resistance to external stress. However, up to now, APXs in Brassica napus (B. napus) have not been systematically characterized. In this study, a total of 26 BnaAPX genes were identified, which were distributed on 13 chromosomes and divided into five phylogenetic branches. Gene structure analysis showed that they had a wide varied number of exons while BnaAPXs proteins contained more similar motifs in the same phylogenetic branches. qRT-PCR analysis of 26 BnaAPX gene expression patterns showed that three putative cytosol BnaAPX genes BnaAPX1, BnaAPX2, BnaAPX9, two putatice microsomal genes BnaAPX18 and BnaAPX25 were up-regulated rapidly and robustly under high salt, water shortage and high temperature stresses. In addition, the above three abiotic stresses led to a significant increase in APX activity. The results provide basic and comprehensive information for further functional characterization of APX gene family in B. napus.
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