Intercalation, DNA Kinking, and the Control of Transcription
Intercalation, DNA Kinking, and the Control of Transcription
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嵌入、DNA 扭结和转录控制
DOI：
10.1126/science.271.5250.778
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发表时间：
1996
期刊：
影响因子：
56.9
通讯作者：
G. Clore
中科院分区：
文献类型：
--
作者：
M. H. Werner;A. Gronenborn;G. Clore
参与转录控制和调节的生物过程依赖于蛋白质诱导的DNA结构畸变，其增强蛋白质向其特异性DNA靶的募集。这一功能通常是由辅助因子完成的，这些辅助因子特异性地结合序列并使DNA局部弯曲或扭结。最近确定的三维结构的几种蛋白质-DNA复合物，涉及蛋白质，执行这样的建筑任务，带来了光的侧链嵌入作为一种机制，能够驱动DNA螺旋的变形的共同主题。定向插入侧链（或多个侧链）的蛋白质支架在结构上是多样的，目前包括可以完成相同任务的四种不同拓扑结构。然而，插入侧链（或多个侧链）仅是疏水性的。插入可以使DNA扭结或弯曲，使一个或多个相邻碱基对解堆积，并使DNA在一整圈螺旋上局部解旋。尽管存在这些扭曲，但B-DNA螺旋参数的恢复通常发生在DNA的相邻半圈内。
Biological processes involved in the control and regulation of transcription are dependent on protein-induced distortions in DNA structure that enhance the recruitment of proteins to their specific DNA targets. This function is often accomplished by accessory factors that bind sequence specifically and locally bend or kink the DNA. The recent determination of the three-dimensional structures of several protein-DNA complexes, involving proteins that perform such architectural tasks, brings to light a common theme of side chain intercalation as a mechanism capable of driving the deformation of the DNA helix. The protein scaffolds orienting the intercalating side chain (or side chains) are structurally diverse, presently comprising four distinct topologies that can accomplish the same task. The intercalating side chain (or side chains), however, is exclusively hydrophobic. Intercalation can either kink or bend the DNA, unstacking one or more adjacent base pairs and locally unwinding the DNA over as much as a full turn of helix. Despite these distortions, the return to B-DNA helical parameters generally occurs within the adjacent half-turns of DNA.
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影响因子：
5.6
作者：
GORIN, AA;ZHURKIN, VB;OLSON, WK
通讯作者：
OLSON, WK
影响因子：
5.6
作者：
Barry D. Starr;Barbara C. Hoopes;Diane K. Hawley
通讯作者：
Barry D. Starr;Barbara C. Hoopes;Diane K. Hawley
TBP 与 TATA 盒的结合诱导了普拉霉素靶向的特定下游解旋位点。
影响因子：
--
作者：
Sun,D;Hurley,LH
通讯作者：
Hurley,LH
TATA 结合蛋白与腺病毒 E4 启动子结合的热力学和动力学表征。
影响因子：
2.9
作者：
Petri,V;Hsieh,M;Brenowitz,M
通讯作者：
Brenowitz,M
DOI：
10.1073/pnas.79.18.5537
发表时间：
1982-09
影响因子：
11.1
作者：
J. Keepers;P. Kollman;P. Weiner;T. James
通讯作者：
J. Keepers;P. Kollman;P. Weiner;T. James