Effects of Antibacterial Peptide F1 on Bacterial Liposome Membrane Integrity.
Effects of Antibacterial Peptide F1 on Bacterial Liposome Membrane Integrity.
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DOI：
10.3389/fnut.2021.768890
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发表时间：
2021
影响因子：
5
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Wang Q;Peng B;Song M;Abdullah;Li J;Miao J;Feng K;Chen F;Zhai X;Cao Y
本实验室前期研究表明，藏灵菇来源的副干酪乳杆菌FX-6发酵乳中获得的抗菌肽F1与普通抗菌肽不同，F1同时抑制革兰氏阴性菌和革兰氏阳性菌的生长。在这里，我们提出了后续的工作表明，抗菌肽F1作用于大肠杆菌ATCC 25922（E。coli）或金黄色葡萄球菌ATCC 63589（S.金黄色葡萄球菌），它们各自的细菌膜严重变形。这种变形使得钾和镁离子从细菌膜中泄漏。通过人工模拟细菌磷脂膜并提取，进一步探讨了抗菌肽F1与细菌膜的相互作用。研究结果表明，抗菌肽F1与细菌细胞膜相互作用后，引起了不同脂质体模拟的钙黄绿素渗漏。透射电子显微镜观察发现，脂质体的磷脂膜结构被破坏，脂质体出现聚集和沉淀。石英晶体微天平（QCM-D）结果表明，抗菌肽F1显着降低脂质体膜的质量和增加其粘弹性。根据研究结果，磷脂膜颗粒尺寸显着增加，表明抗菌肽F1对磷脂膜有直接影响。总之，抗微生物肽F1通过破坏它们各自磷脂膜的共有组分来破坏革兰氏阴性菌和革兰氏阳性菌的膜结构，这导致细胞内容物的泄漏和随后的细胞死亡。
Previous studies from our lab have shown that the antimicrobial peptide F1 obtained from the milk fermentation by Lactobacillus paracasei FX-6 derived from Tibetan kefir was different from common antimicrobial peptides; specifically, F1 simultaneously inhibited the growth of Gram-negative and Gram-positive bacteria. Here, we present follow-on work demonstrating that after the antimicrobial peptide F1 acts on either Escherichia coli ATCC 25922 (E. coli) or Staphylococcus aureus ATCC 63589 (S. aureus), their respective bacterial membranes were severely deformed. This deformation allowed leakage of potassium and magnesium ions from the bacterial membrane. The interaction between the antimicrobial peptide F1 and the bacterial membrane was further explored by artificially simulating the bacterial phospholipid membranes and then extracting them. The study results indicated that after the antimicrobial peptide F1 interacted with the bacterial membranes caused significant calcein leakage that had been simulated by different liposomes. Furthermore, transmission electron microscopy observations revealed that the phospholipid membrane structure was destroyed and the liposomes presented aggregation and precipitation. Quartz Crystal Microbalance with Dissipation (QCM-D) results showed that the antimicrobial peptide F1 significantly reduced the quality of liposome membrane and increased their viscoelasticity. Based on the study's findings, the phospholipid membrane particle size was significantly increased, indicating that the antimicrobial peptide F1 had a direct effect on the phospholipid membrane. Conclusively, the antimicrobial peptide F1 destroyed the membrane structure of both Gram-negative and Gram-positive bacteria by destroying the shared components of their respective phospholipid membranes which resulted in leakage of cell contents and subsequently cell death.
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