Fuc-GM1 ganglioside mimics the receptor function of GM1 for cholera toxin.
Fuc-GM1 ganglioside mimics the receptor function of GM1 for cholera toxin.
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Fuc-GM1 神经节苷脂模拟霍乱毒素 GM1 的受体功能。
DOI：
10.1021/bi00123a030
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Tettamanti,G
中科院分区：
文献类型：
--
作者：
Masserini,M;Freire,E;Palestini,P;Calappi,E;Tettamanti,G
Fuc-GM 1神经节苷脂模拟GM 1对霍乱毒素（CT）的受体功能的能力已经被研究。为此，用Fuc-GMl富集大鼠胶质瘤C6培养细胞，并将对CT的反应性与用GMl神经节苷脂富集的细胞的反应性进行比较。Fuc-GMl被细胞摄取的速度和程度与GMl相同。当神经节苷脂的量相当时，富含Fuc-GM 1的细胞与富含GM 1的细胞结合相同量的125 I-CT。在GM 1-和Fuc-GM 1-富集细胞结合相当量的CT的条件下，Fuc-GM 1-处理的细胞与GM 1-处理的细胞积累几乎相同量的环AMP，并且腺苷酸环化酶的活化也相似。CT诱导的cAMP积累之前的滞后时间在富含Fuc-GM 1和GM 1的细胞中是相同的。高灵敏度等温滴定量热法（ITC）实验表明，CT与Fuc-GM 1或GM 1神经节苷脂的缔合常数相当（25 ℃时分别为4 × 107 M ~ 1和1.9 × 107 M~ 1）。而且，B亚基五聚体与Fuc-GM 1或GM 1神经节苷脂的缔合常数是相当的（在25 ℃下分别为约3 × 107 M-1和7 × 107 M-1）。
Revised Manuscript Received December 5, 1991 abstract: The ability of Fuc-GMl ganglioside to mimic thereceptor function of GM1for cholera toxin (CT) has been investigated. For this purpose, rat glioma C6 cultured cells were enriched with Fuc-GMl and the responsiveness to CT was compared with that of cells enriched with GM1 ganglioside. Fuc-GMl was taken up by cells as rapidly and to the same extent as GM1. Whencomparable amounts of ganglioside were associated, the cells enriched with Fuc-GMl bound the same amount of 125I-CT as did cells enriched with GM1. Underconditions in which GM1-and Fuc-GMl-enriched cells bound comparable amounts of CT, the Fuc-GMl-treated cells accumulated virtually the same amount of cyclic AMP as did GM1-treated cells, and activation of adenylate cyclase was also similar. The lag time preceding the CT-induced cAMP accumulation was the same in Fuc-GMl-and GM1-enriched cells. High-sensitivity isothermal titration calorimetry (ITC) experiments showed that theassociation constants of CT with Fuc-GMl or GM1 ganglioside were comparable (4 X 107 M_1 and 1.9 X 107 M~\respectively, at 25 C). Also, the association constants of the B-subunit pentamer with Fuc-GMl or GM1 ganglioside were comparable (about 3 X 107 M" 1 and 7 X 107 M_1, respectively, at 25 C).
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