Using automated imaging to interrogate gonadotrophin-releasing hormone receptor trafficking and function.
Using automated imaging to interrogate gonadotrophin-releasing hormone receptor trafficking and function.
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DOI：
10.1016/j.mce.2010.07.008
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发表时间：
2011-01-15
影响因子：
4.1
通讯作者：
McArdle, C. A.
中科院分区：
文献类型：
--
作者：
Armstrong, S. P.;Caunt, C. J.;Finch, A. R.;McArdle, C. A.
促性腺激素释放激素（GnRH）通过促性腺激素细胞上的7个跨膜受体刺激促性腺激素的合成和分泌，从而介导生殖的中枢控制。I型哺乳动物GnRHR是独特的，因为它们缺乏C-末端尾巴。这被认为是它们对快速同源脱敏的抗性以及它们缓慢的内化速率和不能引起G蛋白非依赖性（抑制蛋白介导的）信号传导的基础。最近，人们发现绝大多数人GnRHR实际上是细胞内的，尽管它们在细胞表面被膜不渗透肽激素激活。这显然反映了从内质网的低效退出，并且再次，C尾的缺失可能有助于它们的细胞内定位。本综述旨在涵盖GnRHR生物学的一些新方面，重点介绍我们使用自动荧光显微镜（高内容成像）探索GnRHR定位和贩运以及通过Ca 2 +/钙调蛋白/钙调神经磷酸酶/NFAT和Raf/MEK/ERK途径的GnRH信号传导的空间和时间方面。
Gonadotrophin-releasing hormone (GnRH) acts via seven transmembrane receptors on gonadotrophs to stimulate gonadotrophin synthesis and secretion, and thereby mediates central control of reproduction. Type I mammalian GnRHR are unique, in that they lack C-terminal tails. This is thought to underlie their resistance to rapid homologous desensitisation as well as their slow rate of internalisation and inability to provoke G-protein-independent (arrestin-mediated) signalling. More recently it has been discovered that the vast majority of human GnRHR are actually intracellular, in spite of the fact that they are activated at the cell surface by a membrane impermeant peptide hormone. This apparently reflects inefficient exit from the endoplasmic reticulum and again, the absence of the C-tail likely contributes to their intracellular localisation. This review is intended to cover some of these novel aspects of GnRHR biology, focusing on ways that we have used automated fluorescence microscopy (high content imaging) to explore GnRHR localisation and trafficking as well as spatial and temporal aspects of GnRH signalling via the Ca2+/calmodulin/calcineurin/NFAT and Raf/MEK/ERK pathways.
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双重特异性磷酸酶对ERK2的时空调节。
影响因子：
4.8
作者：
Caunt, Christopher J.;Armstrong, Stephen P.;Rivers, Caroline A.;Norman, Michael R.;McArdle, Craig A.
通讯作者：
McArdle, Craig A.
影响因子：
3.5
作者：
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通讯作者：
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影响因子：
56.9
作者：
BELCHETZ, PE;PLANT, TM;KNOBIL, E
通讯作者：
KNOBIL, E
影响因子：
4.8
作者：
DALKIN, AC;HAISENLEDER, DJ;MARSHALL, JC
通讯作者：
MARSHALL, JC
DOI：
10.1073/pnas.96.8.4426
发表时间：
1999-04-13
影响因子：
11.1
作者：
Craske, H;Takeo, T;Tepikin, AV
通讯作者：
Tepikin, AV