Pulmonary injury risk curves and behavioral changes from blast overpressure exposures of varying frequency and intensity in rats.
Pulmonary injury risk curves and behavioral changes from blast overpressure exposures of varying frequency and intensity in rats.
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DOI：
10.1038/s41598-020-73643-7
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发表时间：
2020-10-06
影响因子：
4.6
通讯作者：
Long JB
中科院分区：
文献类型：
--
作者：
Sajja VS;Statz JK;Walker LPB;Gist ID;Wilder DM;Ahlers ST;Long JB
目前，没有一套准则规定军事人员在战斗或训练行动中的爆炸暴露次数和强度的累积限制。本研究的目的是使用先进的冲击波模拟器，在适当的暴露条件和组合(即冲击波超压强度和暴露频率)下，确定原发重复冲击波肺损伤的肺损伤、病理学和相关的行为变化。雄性SD大鼠在 ~ 8.5-19磅/平方英寸的压力范围内，正面和侧面暴露于防喷剂，每天暴露30次。在BOP后24小时，通过评估表面出血/挫伤、苏木精-伊红染色和旷场活动的行为缺陷来确定肺损伤的程度。对肺损伤进行数学建模，以确定军事标准1%肺损伤阈值。在压力 ≥ 10psi时观察到明显的组织细胞增多症和炎症水平，在压力 ≥ 13psi时观察到定向效应。实验数据显示， ~ 8.5psi是出血/挫伤的阈值，最多暴露30次。对数据进行建模，预测了高达50次暴露的伤害风险，其中正面暴露的强度阈值为8psi，侧面暴露的强度阈值为6psi，这需要进一步验证。
At present, there are no set guidelines establishing cumulative limits for blast exposure numbers and intensities in military personnel, in combat or training operations. The objective of the current study was to define lung injury, pathology, and associated behavioral changes from primary repeated blast lung injury under appropriate exposure conditions and combinations (i.e. blast overpressure (BOP) intensity and exposure frequency) using an advanced blast simulator. Male Sprague Dawley rats were exposed to BOP frontally and laterally at a pressure range of ~ 8.5–19 psi, for up to 30 daily exposures. The extent of lung injury was identified at 24 h following BOP by assessing the extent of surface hemorrhage/contusion, Hematoxylin and Eosin staining, and behavioral deficits with open field activity. Lung injury was mathematically modeled to define the military standard 1% lung injury threshold. Significant levels of histiocytosis and inflammation were observed in pressures ≥ 10 psi and orientation effects were observed at pressures ≥ 13 psi. Experimental data demonstrated ~ 8.5 psi is the threshold for hemorrhage/contusion, up to 30 exposures. Modeling the data predicted injury risk up to 50 exposures with intensity thresholds at 8 psi for front exposure and 6psi for side exposures, which needs to be validated further.
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影响因子：
3.4
作者：
Heyburn, Lanier;Abutarboush, Rania;Sajja, Venkata Siva Sai Sujith
通讯作者：
Sajja, Venkata Siva Sai Sujith
DOI：
10.1098/rstb.2010.0251
发表时间：
2011-01-27
影响因子：
6.3
作者：
Smith, J. E.
通讯作者：
Smith, J. E.
影响因子：
3.8
作者：
Elsayed, Nabil M.;Gorbunov, Nikolai V.
通讯作者：
Gorbunov, Nikolai V.
影响因子：
4.2
作者：
Arun, Peethambaran;Wilder, Donna M.;Long, Joseph B.
通讯作者：
Long, Joseph B.
DOI：
10.1097/01.ta.0000233742.75450.47
发表时间：
2006-10-01
影响因子：
--
作者：
Chavko, Mikulas;Prusaczyk, W. Keith;McCarron, Richard M.
通讯作者：
McCarron, Richard M.