A high aspect ratio microelectrode array for mapping neural activity in vitro.
A high aspect ratio microelectrode array for mapping neural activity in vitro.
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DOI：
10.1016/j.jneumeth.2011.11.027
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发表时间：
2012-03-15
影响因子：
3
通讯作者：
Durand, Dominique M.
中科院分区：
文献类型：
--
作者：
Kibler, Andrew B.;Jamieson, Brian G.;Durand, Dominique M.
为了记录神经活动，设计并制作了一种新型的高深宽比穿透微电极阵列。该阵列允许利用高纵横比穿透电极在体外对部位进行二维记录。传统的表面电极阵列虽然容易制造，但不能穿透到有活性的组织，如中央海马片，因此具有比穿透阵列更低的信噪比和选择性。在展开的海马标本中，整个展开的啮齿动物海马标本中的CA1-CA3锥体细胞层被一侧的肺泡和另一侧的Schaffer束包裹，需要穿透电极才能进行高信噪比记录。在透明玻璃衬底上用硅片制作了靶高为200μm、直径为20μm的电极尖峰阵列。测得各电极的阻抗约为1.5MΩ±497KΩ。测量的信噪比为19.4±3分贝，而用压敏染料RH414获得的信号的信噪比为3.9±0.8分贝S/N。在含有50微摩尔4-氨基吡啶的溶液中，用该阵列记录了一个复杂的二维活动波形。这些结果表明，这种新型穿透电极阵列能够获得比电压敏感染料技术更好的数据，用于广域二维神经元活动记录。当与展开的海马体制剂一起使用时，该组合形成了一种独特的功能强大的工具，可以对整个海马体中的海马体网络活动进行成像。
A novel high-aspect-ratio penetrating microelectrode array was designed and fabricated for the purpose of recording neural activity. The array allows two dimensional recording of 64 sites in vitro with high aspect ratio penetrating electrodes. Traditional surface electrode arrays, although easy to fabricate, do not penetrate to the viable tissue such as central hippocampus slices and thus have a lower signal/noise ratio and lower selectivity than a penetrating array. In the unfolded hippocampus preparation, the CA1–CA3 pyramidal cell layer in the whole unfolded rodent hippocampus preparation is encased by the alveus on one side and the Schaffer tract on the other and requires penetrating electrodes for high signal to noise ratio recording. An array of 64 electrode spikes, each with a target height of 200 μm and diameter of 20μm, was fabricated in silicon on a transparent glass substrate. The impedance of the individual electrodes was measured to be approximately 1.5MΩ± 497kΩ. The signal to noise ratio was measured and found to be 19.4 ± 3 dB compared to 3.9 ± 0.8 dB S/N for signals obtained with voltage sensitive dye RH414. A mouse unfolded hippocampus preparation was bathed in solution containing 50 micro-molar 4-Amino Pyridine and a complex two dimensional wave of activity was recorded using the array. These results indicate that this novel penetrating electrode array is able to obtain data superior to that of voltage sensitive dye techniques for broad field two-dimensional neuronal activity recording. When used with the unfolded hippocampus preparation, the combination forms a uniquely capable tool for imaging hippocampal network activity in the entire hippocampus.
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DOI：
10.3389/fneng.2010.00005
发表时间：
2010
期刊：
Frontiers in neuroengineering
影响因子：
--
作者：
Desai SA;Rolston JD;Guo L;Potter SM
通讯作者：
Potter SM
影响因子：
6.1
作者：
Derchansky, M.;Rokni, D.;Carlen, P. L.
通讯作者：
Carlen, P. L.
影响因子：
3.8
作者：
TEYLER, TJ
通讯作者：
TEYLER, TJ
影响因子：
2.9
作者：
Steidl, Esther-Marie;Neueu, Estelle;Buisson, Bruno
通讯作者：
Buisson, Bruno
影响因子：
3
作者：
Ghezzi, Diego;Menegon, Andrea;Ferrigno, Giancarlo
通讯作者：
Ferrigno, Giancarlo