Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis.
Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis.
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DOI：
10.1038/s41467-018-03533-0
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发表时间：
2018-03-16
影响因子：
16.6
通讯作者：
Boulant S
中科院分区：
文献类型：
--
作者：
Bucher D;Frey F;Sochacki KA;Kummer S;Bergeest JP;Godinez WJ;Kräusslich HG;Rohr K;Taraska JW;Schwarz US;Boulant S
尽管对许多细胞过程至关重要，但在网格蛋白介导的内吞过程中，结构和分子事件的顺序仍然难以捉摸。虽然长期以来人们一直认为网格蛋白涂层的凹坑以恒定的曲率生长，但最近有人提出，网格蛋白首先组装形成扁平结构，然后在保持恒定表面积的情况下弯曲。本研究结合相关的电子显微镜和光学显微镜以及数学生长规律，研究了哺乳动物BSC-1细胞内吞过程中网格蛋白外壳的超微结构重排。我们证实，网格蛋白外壳最初是扁平的，并证明当最终的网格蛋白含量达到70%左右时，曲率开始出现。我们发现这种转变的标志是网格蛋白与网格蛋白接头蛋白AP2比值的变化，而膜张力抑制了这种转变。我们的研究结果支持了BSC-1哺乳动物细胞通过生化和机械因素动态调节网格蛋白介导的内吞作用从平面到弯曲的转变的观点。在网格蛋白介导的内吞过程中，结构和分子事件的顺序尚不清楚。在这里，作者结合相关的显微镜和简单的数学生长规律来证明，当最终网格蛋白含量达到70%左右时，平坦的斑块开始弯曲。
Although essential for many cellular processes, the sequence of structural and molecular events during clathrin-mediated endocytosis remains elusive. While it was long believed that clathrin-coated pits grow with a constant curvature, it was recently suggested that clathrin first assembles to form flat structures that then bend while maintaining a constant surface area. Here, we combine correlative electron and light microscopy and mathematical growth laws to study the ultrastructural rearrangements of the clathrin coat during endocytosis in BSC-1 mammalian cells. We confirm that clathrin coats initially grow flat and demonstrate that curvature begins when around 70% of the final clathrin content is acquired. We find that this transition is marked by a change in the clathrin to clathrin-adaptor protein AP2 ratio and that membrane tension suppresses this transition. Our results support the notion that BSC-1 mammalian cells dynamically regulate the flat-to-curved transition in clathrin-mediated endocytosis by both biochemical and mechanical factors. The sequence of structural and molecular events during clathrin-mediated endocytosis is unclear. Here the authors combine correlative microscopy and simple mathematical growth laws to demonstrate that the flat patch starts to curve when around 70% of the final clathrin content is reached.
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DOI：
10.1083/jcb.84.3.560
发表时间：
1980-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser J
通讯作者：
Heuser J
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
3.7
作者：
Boucrot, Emmanuel;Saffarian, Saveez;Ehrlich, Marcelo
通讯作者：
Ehrlich, Marcelo
影响因子：
10.6
作者：
Godinez, William J;Rohr, Karl
通讯作者：
Rohr, Karl
影响因子：
2.4
作者：
Kumar, Gaurav;Sain, Anirban
通讯作者：
Sain, Anirban