LncRNA-mRNA expression profiles and functional networks in osteoclast differentiation.
LncRNA-mRNA expression profiles and functional networks in osteoclast differentiation.
复制标题
破骨细胞分化中的 LncRNA-mRNA 表达谱和功能网络
DOI：
10.1111/jcmm.15560
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发表时间：
2020-09
影响因子：
5.3
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Liu W;Li Z;Cai Z;Xie Z;Li J;Li M;Cen S;Tang S;Zheng G;Ye G;Su H;Wang S;Wang P;Shen H;Wu Y
人破骨细胞由CD 14+单核细胞分化而来，负责骨吸收。长链非编码RNA（lncRNA）已被证明与多种生物学过程，特别是细胞分化密切相关。然而，lncRNA在破骨细胞分化中的作用不太受重视。在我们的研究中，使用RNA测序（RNA-seq）来鉴定破骨细胞分化中lncRNA和mRNA的表达谱。结果表明，破骨细胞分化后，1117个lncRNA和296个mRNA的表达发生了显著变化。进行qRT-PCR测定以确认表达谱，结果与RNA-seq数据几乎一致。GO和KEGG分析用于预测这些差异表达的mRNA和lncRNA的功能。Path‐net分析表明MAPK通路、PI 3 K-AKT通路和NF-κ B B通路在破骨细胞分化中起重要作用。共表达网络和竞争性内源性RNA网络表明ENSG00000257764.2-miR-106 a-5 p-TIMP 2可能在破骨细胞分化中发挥核心作用。我们的研究为进一步了解lncRNA在破骨细胞分化中的作用和机制奠定了基础，其中许多lncRNA可能是骨代谢疾病的潜在靶点。
Human osteoclasts are differentiated from CD14+ monocytes and are responsible for bone resorption. Long non‐coding RNAs (lncRNAs) have been proved to be significantly involved in multiple biologic processes, especially in cell differentiation. However, the effect of lncRNAs in osteoclast differentiation is less appreciated. In our study, RNA sequencing (RNA‐seq) was used to identify the expression profiles of lncRNAs and mRNAs in osteoclast differentiation. The results demonstrated that expressions of 1117 lncRNAs and 296 mRNAs were significantly altered after osteoclast differentiation. qRT‐PCR assays were performed to confirm the expression profiles, and the results were almost consistent with the RNA‐seq data. GO and KEGG analyses were used to predict the functions of these differentially expressed mRNA and lncRNAs. The Path‐net analysis demonstrated that MAPK pathway, PI3K‐AKT pathway and NF‐kappa B pathway played important roles in osteoclast differentiation. Co‐expression networks and competing endogenous RNA networks indicated that ENSG00000257764.2‐miR‐106a‐5p‐TIMP2 may play a central role in osteoclast differentiation. Our study provides a foundation to further understand the role and underlying mechanism of lncRNAs in osteoclast differentiation, in which many of them could be potential targets for bone metabolic disease.
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影响因子：
12.7
作者：
Bartelt A;Behler-Janbeck F;Beil FT;Koehne T;Müller B;Schmidt T;Heine M;Ochs L;Yilmaz T;Dietrich M;Tuckermann JP;Amling M;Herz J;Schinke T;Heeren J;Niemeier A
通讯作者：
Niemeier A
影响因子：
82.9
作者：
Dankbar, Berno;Fennen, Michelle;Pap, Thomas
通讯作者：
Pap, Thomas
lncRNA HOTAIRM1通过增强自噬途径调节PML-RARA癌蛋白的降解和骨髓细胞分化
影响因子：
12.4
作者：
Chen ZH;Wang WT;Huang W;Fang K;Sun YM;Liu SR;Luo XQ;Chen YQ
通讯作者：
Chen YQ
影响因子：
29
作者：
Kim H;Kim T;Jeong BC;Cho IT;Han D;Takegahara N;Negishi-Koga T;Takayanagi H;Lee JH;Sul JY;Prasad V;Lee SH;Choi Y
通讯作者：
Choi Y
影响因子：
13.3
作者：
Laha, Dipranjan;Deb, Moonmoon;Das, Hiranmoy
通讯作者：
Das, Hiranmoy