Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications.
Advanced Flow Cytometry Assays for Immune Monitoring of CAR-T Cell Applications.
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用于免疫监测CAR-T细胞应用的晚期流式细胞仪测定法。
DOI：
10.3389/fimmu.2021.658314
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发表时间：
2021
影响因子：
7.3
通讯作者：
Koehl U
中科院分区：
文献类型：
--
作者：
Blache U;Weiss R;Boldt A;Kapinsky M;Blaudszun AR;Quaiser A;Pohl A;Miloud T;Burgaud M;Vucinic V;Platzbecker U;Sack U;Fricke S;Koehl U
Adoptive immunotherapy using chimeric antigen receptor (CAR)-T cells has achieved successful remissions in refractory B-cell leukemia and B-cell lymphomas. In order to estimate both success and severe side effects of CAR-T cell therapies, longitudinal monitoring of the patient’s immune system including CAR-T cells is desirable to accompany clinical staging. To conduct research on the fate and immunological impact of infused CAR-T cells, we established standardized 13-colour/15-parameter flow cytometry assays that are suitable to characterize immune cell subpopulations in the peripheral blood during CAR-T cell treatment. The respective staining technology is based on pre-formulated dry antibody panels in a uniform format. Additionally, further antibodies of choice can be added to address specific clinical or research questions. We designed panels for the anti-CD19 CAR-T therapy and, as a proof of concept, we assessed a healthy individual and three B-cell lymphoma patients treated with anti-CD19 CAR-T cells. We analyzed the presence of anti-CD19 CAR-T cells as well as residual CD19+ B cells, the activation status of the T-cell compartment, the expression of co-stimulatory signaling molecules and cytotoxic agents such as perforin and granzyme B. In summary, this work introduces standardized and modular flow cytometry assays for CAR-T cell clinical research, which could also be adapted in the future as quality controls during the CAR-T cell manufacturing process.
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影响因子：
3.7
作者：
Lee, Jamie A.;Spidlen, Josef;Boyce, Keith;Cai, Jennifer;Crosbie, Nicholas;Dalphin, Mark;Furlong, Jeff;Gasparetto, Maura.;Goldberg, Michael;Goralczyk, Elizabeth M.;Hyun, Bill;Jansen, Kirstin;Kollmann, Tobias;Kong, Megan;Leif, Robert;McWeeney, Shannon;Moloshok, Thomas D.;Moore, Wayne;Nolan, Garry;Nolan, John;Nikolich-Zugich, Janko;Parrish, David;Purcell, Barclay;Qian, Yu;Selvaraj, Biruntha;Smith, Clayton;Tchuvatkina, Olga;Wertheimer, Anne;WilkinSon, Peter;Wilson, Christopher;Wood, James;Zigon, Robert;Scheuermann, Richard H.;Brinkman, Ryan R.
通讯作者：
Brinkman, Ryan R.
影响因子：
7.3
作者：
Mehta RS;Rezvani K
通讯作者：
Rezvani K
影响因子：
82.9
作者：
Deng Q;Han G;Puebla-Osorio N;Ma MCJ;Strati P;Chasen B;Dai E;Dang M;Jain N;Yang H;Wang Y;Zhang S;Wang R;Chen R;Showell J;Ghosh S;Patchva S;Zhang Q;Sun R;Hagemeister F;Fayad L;Samaniego F;Lee HC;Nastoupil LJ;Fowler N;Eric Davis R;Westin J;Neelapu SS;Wang L;Green MR
通讯作者：
Green MR
影响因子：
46.9
作者：
Klichinsky M;Ruella M;Shestova O;Lu XM;Best A;Zeeman M;Schmierer M;Gabrusiewicz K;Anderson NR;Petty NE;Cummins KD;Shen F;Shan X;Veliz K;Blouch K;Yashiro-Ohtani Y;Kenderian SS;Kim MY;O'Connor RS;Wallace SR;Kozlowski MS;Marchione DM;Shestov M;Garcia BA;June CH;Gill S
通讯作者：
Gill S
DOI：
10.1056/nejmoa1407222
发表时间：
2014-10-16
期刊：
The New England journal of medicine
影响因子：
--
作者：
Maude SL;Frey N;Shaw PA;Aplenc R;Barrett DM;Bunin NJ;Chew A;Gonzalez VE;Zheng Z;Lacey SF;Mahnke YD;Melenhorst JJ;Rheingold SR;Shen A;Teachey DT;Levine BL;June CH;Porter DL;Grupp SA
通讯作者：
Grupp SA