MIFlowCyt: the minimum information about a Flow Cytometry Experiment.

MIFlowCyt: the minimum information about a Flow Cytometry Experiment.
复制标题

DOI:
10.1002/cyto.a.20623
复制
发表时间:
2008-10
期刊:
影响因子:
3.7
通讯作者:
Brinkman, Ryan R.
Brinkman, Ryan R.
中科院分区:
生物学4区
文献类型:
--
作者:
Lee, Jamie A.;Spidlen, Josef;Boyce, Keith;Cai, Jennifer;Crosbie, Nicholas;Dalphin, Mark;Furlong, Jeff;Gasparetto, Maura.;Goldberg, Michael;Goralczyk, Elizabeth M.;Hyun, Bill;Jansen, Kirstin;Kollmann, Tobias;Kong, Megan;Leif, Robert;McWeeney, Shannon;Moloshok, Thomas D.;Moore, Wayne;Nolan, Garry;Nolan, John;Nikolich-Zugich, Janko;Parrish, David;Purcell, Barclay;Qian, Yu;Selvaraj, Biruntha;Smith, Clayton;Tchuvatkina, Olga;Wertheimer, Anne;WilkinSon, Peter;Wilson, Christopher;Wood, James;Zigon, Robert;Scheuermann, Richard H.;Brinkman, Ryan R.

文献摘要

参考文献

被引文献

相似文献

科学研究的一个基本原则是,发表的结果是开放的独立验证和反驳。最低数据标准通过提供明确解释实验结果所需的规范,帮助数据提供者、用户和出版商。在这里,我们提出了关于流式细胞术实验(MIFlowCyt)标准的最低信息,说明了报告流式细胞术(FCM)实验所需的最低信息。我们召集了一个跨学科的国际合作小组,由生物信息学家、计算统计学家、软件开发人员、仪器制造商以及临床和基础研究科学家组成,以制定该标准。该标准随后由国际细胞计数促进协会(ISAC)数据标准工作组、标准委员会、成员和理事会审查。MIFlowCyt标准包括有关FCM实验中包含的标本和试剂的描述、用于执行测定的仪器配置以及用于解释主要输出数据的数据处理方法的建议。MIFlowCyt已被ISAC采纳为标准,代表流式细胞术科学界,包括科学家以及软件和硬件制造商。MIFlowCyt被科学界和出版界采用将促进第三方对流式细胞术数据的理解和重用。
A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for the Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the flow cytometry scientific community including scientists as well as software and hardware manufactures. MIFlowCyt’s adoption by the scientific and publishing communities will facilitate third-party understanding and reuse of flow cytometry data.
DOI: 10.1038/nbt1156
发表时间: 2005-12-01
影响因子: 46.9
作者:
Le Novère, N;Finney, A;Wanner, BL
通讯作者: Wanner, BL
DOI: 10.1186/1471-2172-6-13
发表时间: 2005-06-24
期刊: BMC IMMUNOLOGY
影响因子: 3
作者:
Maecker, HT;Rinfret, A;Sekaly, RP
通讯作者: Sekaly, RP
DOI: 10.1016/j.jim.2004.06.003
发表时间: 2004-09-01
影响因子: 2.2
作者:
Gasparetto, M;Gentry, T;Smith, C
通讯作者: Smith, C
DOI: 10.1038/nbt.1411
发表时间: 2008-08
影响因子: 46.9
作者:
Taylor, Chris F.;Field, Dawn;Sansone, Susanna-Assunta;Aerts, Jan;Apweiler, Rolf;Ashburner, Michael;Ball, Catherine A.;Binz, Pierre-Alain;Bogue, Molly;Booth, Tim;Brazma, Alvis;Brinkman, Ryan R.;Clark, Adam Michael;Deutsch, Eric W.;Fiehn, Oliver;Fostel, Jennifer;Ghazal, Peter;Gibson, Frank;Gray, Tanya;Grimes, Graeme;Hancock, John M.;Hardy, Nigel W.;Hermjakob, Henning;Julian, Randall K., Jr.;Kane, Matthew;Kettner, Carsten;Kinsinger, Christopher;Kolker, Eugene;Kuiper, Martin;Le Novere, Nicolas;Leebens-Mack, Jim;Lewis, Suzanna E.;Lord, Phillip;Mallon, Ann-Marie;Marthandan, Nishanth;Masuya, Hiroshi;McNally, Ruth;Mehrle, Alexander;Morrison, Norman;Orchard, Sandra;Quackenbush, John;Reecy, James M.;Robertson, Donald G.;Rocca-Serra, Philippe;Rodriguez, Henry;Rosenfelder, Heiko;Santoyo-Lopez, Javier;Scheuermann, Richard H.;Schober, Daniel;Smith, Barry;Snape, Jason;Stoeckert, Christian J., Jr.;Tipton, Keith;Sterk, Peter;Untergasser, Andreas;Vandesompele, Jo;Wiemann, Stefan
通讯作者: Wiemann, Stefan
DOI: 10.1089/omi.2006.10.209
发表时间: 2006-06-01
影响因子: 3.3
作者:
Spidlen, Josef;Gentleman, Robert C.;Brinkman, Ryan R.
通讯作者: Brinkman, Ryan R.