Screening of Oligomeric (Meth)acrylate Vaccine Adjuvants Synthesized via Catalytic Chain Transfer Polymerization.
Screening of Oligomeric (Meth)acrylate Vaccine Adjuvants Synthesized via Catalytic Chain Transfer Polymerization.
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DOI：
10.3390/polym15183831
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发表时间：
2023-09-20
期刊：
影响因子：
5
通讯作者：
Irvine DJ
中科院分区：
文献类型：
--
作者：
Hege CS;Stimpson A;Sefton J;Summers J;Henke H;Dundas AA;Phan T;Kinsey R;Guderian JA;Sivananthan SJ;Mohamath R;Lykins WR;Ramer-Denisoff G;Lin S;Fox CB;Irvine DJ
该报告详细介绍了自由基产生的甲基丙烯酸酯低聚物反应混合物作为替代疫苗佐剂成分的首次系统筛选，以取代目前的基准化合物角鲨烯，该化合物不可持续地来源于鲨鱼肝脏。均聚物/共聚低聚物的甲基，丁基，月桂基，和硬脂基甲基丙烯酸酯的混合物成功地合成使用催化链转移控制，其中使用微波加热被证明是促进链转移的传播。通过控制混合物的材料特性，可以获得正确的粘度，从而使混合物能够有效地用于疫苗制剂。当与人全血一起孵育时，所选寡聚物的乳液刺激与角鲨烯乳液相当的细胞因子水平，并且当与灭活流感疫苗一起施用时，引发抗原特异性细胞免疫应答，表明化合物作为疫苗佐剂组分的潜在效用。此外，低聚物的分子尺寸被证明足够大以使得能够实现比角鲨烯更大的乳液稳定性，特别是在高温下，但预计足够小以允许从身体快速清除。
This report details the first systematic screening of free-radical-produced methacrylate oligomer reaction mixtures as alternative vaccine adjuvant components to replace the current benchmark compound squalene, which is unsustainably sourced from shark livers. Homo-/co-oligomer mixtures of methyl, butyl, lauryl, and stearyl methacrylate were successfully synthesized using catalytic chain transfer control, where the use of microwave heating was shown to promote propagation over chain transfer. Controlling the mixture material properties allowed the correct viscosity to be achieved, enabling the mixtures to be effectively used in vaccine formulations. Emulsions of selected oligomers stimulated comparable cytokine levels to squalene emulsion when incubated with human whole blood and elicited an antigen-specific cellular immune response when administered with an inactivated influenza vaccine, indicating the potential utility of the compounds as vaccine adjuvant components. Furthermore, the oligomers’ molecular sizes were demonstrated to be large enough to enable greater emulsion stability than squalene, especially at high temperatures, but are predicted to be small enough to allow for rapid clearance from the body.
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DOI：
10.1016/s0950-4230(01)00070-5
发表时间：
2002-05-01
影响因子：
3.5
作者：
Kao, CS;Hu, KH
通讯作者：
Hu, KH
影响因子：
5.5
作者：
Hilgers, LAT;Nicolas, I;Boon, B
通讯作者：
Boon, B
影响因子：
4.8
作者：
Pavot V;Bisceglia H;Guillaume F;Montano S;Zhang L;Boudet F;Haensler J
通讯作者：
Haensler J
影响因子：
4.6
作者：
Allan, Laura E. N.;Shaver, Michael P.;Gibson, Vernon C.
通讯作者：
Gibson, Vernon C.
影响因子：
7.8
作者：
Faruck, Mohammad Omer;Zhao, Lili;Toth, Istvan
通讯作者：
Toth, Istvan