Co-expression of CXCR4 and CXCR7 in human endometrial stromal cells is modulated by steroid hormones.
Co-expression of CXCR4 and CXCR7 in human endometrial stromal cells is modulated by steroid hormones.
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CXCR4 和 CXCR7 在人子宫内膜基质细胞中的共表达受到类固醇激素的调节。
DOI：
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Mei-Rong Du
中科院分区：
文献类型：
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作者：
Wen-Hui Zhou;Xia Wu;Wei-Dong Hu;Mei-Rong Du
子宫内膜受雌激素(E)和孕酮(P)的调节，对着床和妊娠具有重要作用。本研究采用免疫组织化学方法比较了趋化因子CXCL12及其受体CXCR4和CXCR7在人周期子宫内膜和早孕子宫内膜中的表达。从基因水平和蛋白水平探讨E和P对人子宫内膜间质细胞CXCL12、CXCR4和CXCR7表达的调节作用。免疫组化结果显示，月经期人胚胎干细胞不表达CXCL12、CXCR4和CXCR7蛋白，而在增殖期和分泌期表达CXCR4和CXCR7而不表达CXCL12，且在妊娠早期表达强度最高。E和P均能上调ESCs中CXCR4和CXCR7的表达(P<；0.01)。因此，ESCs在时空上共表达CXCR4和CXCR7而不是CXCL12，并且E和P能够调节ESCs中CXCR4和CXCR7的表达，提示类固醇激素对ESCs趋化因子受体表达的调节。
Endometrium modulated by estrogen (E) and progesterone (P) is important for implantation and pregnancy. The present study compared the expression of chemokine CXCL12 and chemokine receptor CXCR4 and CXCR7 between human cycling and early pregnant endometria by immunohistochemistry (IHC). Then the modulation of E and P on expression of CXCL12, CXCR4 and CXCR7 in human endometrial stromal cells (ESCs) was explored at both mRNA and protein level. The result of IHC showed that human ESCs of the menstrual period did not express CXCL12, CXCR4 or CXCR7 protein, however, the expression of CXCR4 and CXCR7 but not CXCL12 in ESCs increased in the proliferative and secretory phase, and the expression intensity for CXCR4 and CXCR7 in ESCs was the highest in the first trimester. Moreover, E and P were able to up-regulate the mRNA and protein expression of CXCR4 and protein expression of CXCR7 in ESCs (P<0.01). Thus, ESCs spatiotemporally co-express CXCR4 and CXCR7 rather than CXCL12, and E and P are able to regulate the expression of CXCR4 and CXCR7 in ESCs, suggesting the modulation of steroid hormones on chemokine receptor expression in ESCs.
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影响因子：
3.7
作者：
Teklenburg G;Salker M;Molokhia M;Lavery S;Trew G;Aojanepong T;Mardon HJ;Lokugamage AU;Rai R;Landles C;Roelen BA;Quenby S;Kuijk EW;Kavelaars A;Heijnen CJ;Regan L;Brosens JJ;Macklon NS
通讯作者：
Macklon NS
趋化因子 CXCL12 促进人类妊娠早期滋养层细胞和蜕膜基质细胞之间的相互作用。
影响因子：
6.1
作者：
Zhou, Wen-Hui;Du, Mei-Rong;Li, Da-Jin
通讯作者：
Li, Da-Jin
影响因子：
9.1
作者：
V. Tripathi;R. Verma;A. Dinda;N. Malhotra;J. Kaur;K. Luthra
通讯作者：
V. Tripathi;R. Verma;A. Dinda;N. Malhotra;J. Kaur;K. Luthra
影响因子：
3.6
作者：
Hess, A. P.;Hamilton, A. E.;Giudice, L. C.
通讯作者：
Giudice, L. C.
影响因子：
3.6
作者：
K. Yoshinaga
通讯作者：
K. Yoshinaga