Surprisingly simple mechanical behavior of a complex embryonic tissue.
Surprisingly simple mechanical behavior of a complex embryonic tissue.
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DOI：
10.1371/journal.pone.0015359
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发表时间：
2010-12-28
期刊：
影响因子：
3.7
通讯作者：
Davidson LA
中科院分区：
文献类型：
--
作者：
von Dassow M;Strother JA;Davidson LA
先前的研究表明，机械反馈可以协调胚胎的形态发生事件。此外，胚胎组织具有复杂的结构和组成，并在形态发生过程中发生较大的变形。因此，我们期望胚胎的组织力学特性高度非线性和加载率相关。我们用微吸法测试了一个简单的线性粘弹性模型是否足以描述非洲爪原胚期胚胎组织在体内的力学行为。我们测试了这些胚胎组织是否会在机械刺激下改变其机械性能，但没有发现组织的粘弹性性能在响应应力或应力施加率时发生变化的证据。我们使用这个模型来检验关于电诱导组织收缩过程中力产生模式的假设。收缩对吸力压力的依赖与根尖张力最一致，而与各向同性收缩不一致。最后，更硬的离合器产生更强的收缩，这表明力的产生和刚度可能在胚胎中耦合。一个复杂的，活跃的胚胎组织的力学行为可以很好地描述一个简单的线性粘弹性模型与幂律蠕变顺应，即使在高变形。我们在这个系统中没有发现机械反馈的证据。总之，这些结果表明，非常简单的力学模型可以用于描述胚胎力学。
Previous studies suggest that mechanical feedback could coordinate morphogenetic events in embryos. Furthermore, embryonic tissues have complex structure and composition and undergo large deformations during morphogenesis. Hence we expect highly non-linear and loading-rate dependent tissue mechanical properties in embryos. We used micro-aspiration to test whether a simple linear viscoelastic model was sufficient to describe the mechanical behavior of gastrula stage Xenopus laevis embryonic tissue in vivo. We tested whether these embryonic tissues change their mechanical properties in response to mechanical stimuli but found no evidence of changes in the viscoelastic properties of the tissue in response to stress or stress application rate. We used this model to test hypotheses about the pattern of force generation during electrically induced tissue contractions. The dependence of contractions on suction pressure was most consistent with apical tension, and was inconsistent with isotropic contraction. Finally, stiffer clutches generated stronger contractions, suggesting that force generation and stiffness may be coupled in the embryo. The mechanical behavior of a complex, active embryonic tissue can be surprisingly well described by a simple linear viscoelastic model with power law creep compliance, even at high deformations. We found no evidence of mechanical feedback in this system. Together these results show that very simple mechanical models can be useful in describing embryo mechanics.
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影响因子：
64.5
作者：
Choquet, D;Felsenfeld, DP;Sheetz, MP
通讯作者：
Sheetz, MP
影响因子：
3.4
作者：
Kasza, K. E.;Nakamura, F.;Weitz, D. A.
通讯作者：
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影响因子：
3.8
作者：
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通讯作者：
Sato, M
DOI：
10.1016/j.cub.2009.06.044
发表时间：
2009-08-25
期刊：
Current biology : CB
影响因子：
--
作者：
Clark AG;Miller AL;Vaughan E;Yu HY;Penkert R;Bement WM
通讯作者：
Bement WM
影响因子：
2.4
作者：
Fabry, B;Maksym, GN;Fredberg, JJ
通讯作者：
Fredberg, JJ