Integration of single and multicellular wound responses.
Integration of single and multicellular wound responses.
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DOI：
10.1016/j.cub.2009.06.044
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发表时间：
2009-08-25
期刊：
影响因子：
--
通讯作者：
Bement WM
中科院分区：
文献类型：
--
作者：
Clark AG;Miller AL;Vaughan E;Yu HY;Penkert R;Bement WM
单细胞和多细胞组织能迅速愈合伤口。这些过程被认为是不同的，但一种愈合模式-Rho GTP酶依赖性的形成和闭合的荷包缝合的肌动蛋白丝（F-肌动蛋白）和肌球蛋白-2伤口周围-发生在单细胞和上皮细胞。在这里，我们表明，创伤的一个细胞在非洲爪蟾胚胎elaimed Rho GTdR激活伤口周围，并在最近的细胞连接在相邻的细胞。F-肌动蛋白和肌球蛋白-2在连接处以及伤口本身周围积累，并且随着所得的肌动球蛋白阵列在伤口部位上闭合，连接的F-肌动蛋白和肌球蛋白-2与伤口周围的肌动蛋白和肌球蛋白-2机械地整合，形成混合荷包线。当细胞被消融而不是受伤时，Rho GT3激活和F-肌动蛋白积聚发生在消融细胞周围的细胞-细胞连接处，并且荷包缝合闭合上皮中的孔。细胞内游离钙的升高，一个重要的上游信号的单细胞伤口反应，也发生在细胞-细胞接触和相邻细胞。因此，单细胞和多细胞荷包缝合伤口反应代表了信号传导和机械连续体上的点，这些点通过细胞-细胞连接整合。
Single cells and multicellular tissues rapidly heal wounds. These processes are considered distinct, but one mode of healing—Rho GTPase-dependent formation and closure of a purse string of actin filaments (F-actin) and myosin-2 around wounds—occurs in single cells and in epithelia. Here we show that wounding of one cell in Xenopus embryos elicits Rho GTPase activation around the wound and at the nearest cell-cell junctions in the neighbor cells. F-actin and myosin-2 accumulate at the junctions as well as around the wound itself, and as the resultant actomyosin array closes over the wound site, junctional F-actin and myosin-2 become mechanically integrated with the actin and myosin-2 around the wound, forming a hybrid purse string. When cells are ablated rather than wounded, Rho GTPase activation and F-actin accumulation occur at cell-cell junctions surrounding the ablated cell, and the purse string closes the hole in the epithelium. Elevation of intracellular free calcium, an essential upstream signal for the single cell wound response, also occurs at the cell-cell contacts and in neighbor cells. Thus, the single and multicellular purse string wound responses represent points on a signaling and mechanical continuum that are integrated by cell-cell junctions.
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影响因子：
64.8
作者：
MARTIN, P;LEWIS, J
通讯作者：
LEWIS, J
影响因子：
7.8
作者：
Brock, J;Midwinter, K;Martin, P
通讯作者：
Martin, P
DOI：
10.1083/jcb.200609116
发表时间：
2007-01-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Tamada M;Perez TD;Nelson WJ;Sheetz MP
通讯作者：
Sheetz MP
影响因子：
--
作者：
Burkel, Brian M.;von Dassow, George;Bement, William M.
通讯作者：
Bement, William M.
影响因子：
64.5
作者：
Oancea, E;Meyer, T
通讯作者：
Meyer, T