Recognition and cleavage of a nonstructured CRISPR RNA by its processing endoribonuclease Cas6.
Recognition and cleavage of a nonstructured CRISPR RNA by its processing endoribonuclease Cas6.
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DOI：
10.1016/j.str.2013.01.010
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发表时间：
2013-03-05
期刊：
影响因子：
5.7
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Shao, Yaming;Li, Hong
CRISPR（Regularly Interspaced Short Palindromic Repeats）通过小RNA介导的机制赋予原核生物适应性免疫。所有携带CRISPR的生物体都需要特异性内切核糖核酸酶将CRISPR RNA加工成小RNA，作为防御效应复合物的向导。核糖核酸内切酶如何加工不含二级结构特征的CRISPR RNA的分子机制在很大程度上仍然难以捉摸。在这里，我们报告的共晶体结构的加工内切核糖核酸酶绑定与不可切割的RNA基板和其产品的类似片段来自nonpalindramic重复。该酶稳定切割位点附近的短RNA茎环结构，并使用由精氨酸和赖氨酸残基组成的活性位点切割磷酸二酯键。不同的RNA结合和切割机制强调了CRISPR RNA加工的多样性。
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no secondary structural features remains largely elusive. Here we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing.
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DOI：
10.1038/nrg2749
发表时间：
2010-03
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
2.8
作者：
Pourcel, C;Salvignol, G;Vergnaud, G
通讯作者：
Vergnaud, G
影响因子：
4.5
作者：
Hale, Caryn;Kleppe, Kyle;Terns, Michael P.
通讯作者：
Terns, Michael P.
影响因子：
3.9
作者：
Mojica, FJM;Díez-Villaseñor, C;Soria, E
通讯作者：
Soria, E