Single-molecule four-color FRET.
Single-molecule four-color FRET.
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DOI:
10.1002/anie.201005402
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发表时间:
2010-12-17
影响因子:
16.6
通讯作者:
Hohng, Sungchul
中科院分区:
文献类型:
--
作者:
Lee, Jinwoo;Lee, Sanghwa;Ragunathan, Kaushik;Joo, Chirlmin;Ha, Taekjip;Hohng, Sungchul
We developed a single-molecule four-color FRET technique both in confocal and in total-internal-reflection fluorescence microscopies. Real-time determination of six inter-fluorophore FRET efficiencies allowed us to probe the correlated motion of four arms of the Holliday junction. The technique was also applied to assess the correlation of RecA-mediated strand exchange events at both ends of a synaptic complex.
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