Ultrastructural alterations of endothelium covering advanced atherosclerotic plaque in human carotid artery visualised by scanning electron microscope.
Ultrastructural alterations of endothelium covering advanced atherosclerotic plaque in human carotid artery visualised by scanning electron microscope.
复制标题
通过扫描电子显微镜观察人颈动脉中晚期动脉粥样硬化斑块内皮细胞的超微结构改变。
DOI：
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发表时间：
2002
影响因子：
2.2
通讯作者：
M. Frontczak
中科院分区：
文献类型：
--
作者：
Michał Walski;Stefan Chłopicki;R. Celary;M. Frontczak
人类动脉粥样硬化斑块形态在其各个阶段广泛记录使用光学显微镜。然而，对人类动脉粥样硬化斑块的超微结构，特别是动脉粥样硬化中内皮细胞的超微结构知之甚少。本文应用扫描电镜观察了颈动脉粥样硬化斑块表面内皮细胞的变化。检查标本进行动脉粥样硬化病变的颈内动脉，收集8例谁经历了动脉内膜切除术。我们发现动脉粥样硬化斑块的管腔表面有广泛的病理改变。在血管的主要部分，内皮层被保留，但在内皮结构中显示出明显的不规则性，包括立方细胞的外观。一些内皮细胞被许多微绒毛覆盖和/或含有破坏内皮连续表面的"火山口"。经常观察到血小板和白细胞粘附于内皮。还有血管腔区域存在内皮剥脱，其中可见含有纤维蛋白和胶原纤维的内皮下表面。有趣的是，观察到倾向于覆盖部分裸露血管的内皮细胞增殖的迹象。总之，在扫描电子显微镜下，晚期动脉粥样硬化斑块中保存的内皮细胞显示出明显的病理学变化;这些变化是否代表内皮功能障碍的超微结构相关性仍有待确定。
Human atherosclerotic plaque morphology at its various stages was extensively documented using light microscopy. However, much less is known of the ultrastructure of the human atherosclerotic plaque, in particular of ultrastructure of endothelial cells in atherosclerosis. Here, we analysed alterations of endothelial cells covering advanced atherosclerotic plaque in carotid artery using scanning electron microscope. Examination was performed on specimens from atherosclerotic lesions of the interior carotid artery, collected from 8 patients who had undergone endarterectomy. We found wide spectrum of pathological alterations of the luminal surface of atherosclerotic plaque. In dominant part of the vessel, endothelial layer was preserved but displayed pronounced irregularities in endothelial architecture including appearance of cuboidal cells. Some endothelial cells were covered by numerous microvilli and/or contained "craters" disrupting continuous surface of the endothelium. Platelets and leukocytes adhering to endothelium were frequently observed. There were also areas of the vessel lumen with endothelial denudation, in which the subendothelial surface containing fibrin proteins and collagen fibrils were visible. Interestingly, signs of proliferation of endothelial cells tending to cover the partially denuded vessel were observed. In summary, in scanning electron microscope, preserved endothelial cells of advanced atherosclerotic plaque displayed pronounced pathology; whether any of these changes represent the ultrastructural correlate of endothelial dysfunction remains to be established.
动脉损伤后细胞增殖的动力学。
DOI：
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发表时间：
1986
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Clowes,AW;Clowes,MM;Reidy,MA
通讯作者：
Reidy,MA
影响因子：
8.7
作者：
Virmani, R;Kolodgie, FD;Schwartz, SM
通讯作者：
Schwartz, SM
实验性泡沫细胞损伤内皮的保存和结构适应。
DOI：
10.1161/01.atv.9.6.881
发表时间：
1989
期刊：
Arteriosclerosis (Dallas, Tex.)
影响因子：
--
作者：
Taylor,KE;Glagov,S;Zarins,CK
通讯作者：
Zarins,CK