Panax notoginseng Root Cell Death Caused by the Autotoxic Ginsenoside Rg(1) Is Due to Over-Accumulation of ROS, as Revealed by Transcriptomic and Cellular Approaches.
Panax notoginseng Root Cell Death Caused by the Autotoxic Ginsenoside Rg(1) Is Due to Over-Accumulation of ROS, as Revealed by Transcriptomic and Cellular Approaches.
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转录组学和细胞学方法揭示，自毒性人参皂苷 Rg1 引起的三七根细胞死亡是由于 ROS 过度积累所致
DOI：
10.3389/fpls.2018.00264
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhu S
中科院分区：
文献类型：
--
作者：
Yang M;Chuan Y;Guo C;Liao J;Xu Y;Mei X;Liu Y;Huang H;He X;Zhu S
三七是一种价值很高的药用植物，但其栽培受到移栽失败的强烈阻碍，主要是由于自毒作用。破译植物对自毒素的反应机制对于克服观察到的自毒至关重要。在这里，我们通过转录和细胞方法阐明了三七对自毒人参皂苷Rg1的反应。细胞分析表明，Rg1通过破坏细胞膜和细胞壁来抑制根的生长。转录分析证实，与细胞膜、细胞壁分解和ROS代谢相关的基因在Rg1胁迫下表达上调。进一步的细胞分析表明，Rg1通过抑制抗坏血酸过氧化物酶(APX)和参与抗坏血酸-谷胱甘肽(ASC-GSH)循环的酶的活性而诱导根细胞中ROS(O2·-和H2O2)的积累。外源抗氧化剂(ASC和龙胆二糖)通过促进超氧化物歧化酶(SOD)活性和ASC-GSH循环，帮助细胞清除过度积累的ROS。总的来说，自体毒素Rg1通过诱导ROS的过度积累而导致根细胞死亡，而外源抗氧化剂的使用可能是克服自毒的一种策略。
Panax notoginseng is a highly valuable medicinal herb, but its culture is strongly hindered by replant failure, mainly due to autotoxicity. Deciphering the response mechanisms of plants to autotoxins is critical for overcoming the observed autotoxicity. Here, we elucidated the response of P. notoginseng to the autotoxic ginsenoside Rg1 via transcriptomic and cellular approaches. Cellular analyses demonstrated that Rg1 inhibited root growth by disrupting the cell membrane and wall. Transcriptomic analyses confirmed that genes related to the cell membrane, cell wall decomposition and reactive oxygen species (ROS) metabolism were up-regulated by Rg1 stress. Further cellular analyses revealed that Rg1 induced ROS (O2·- and H2O2) accumulation in root cells by suppressing ascorbate peroxidase (APX) and the activities of enzymes involved in the ascorbate-glutathione (ASC-GSH) cycle. Exogenous antioxidants (ASC and gentiobiose) helped cells scavenge over-accumulated ROS by promoting superoxide dismutase (SOD) activity and the ASC-GSH cycle. Collectively, the autotoxin Rg1 caused root cell death by inducing the over-accumulation of ROS, and the use of exogenous antioxidants could represent a strategy for overcoming autotoxicity.
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影响因子：
4.1
作者：
FRY, SC;SMITH, RC;MATTHEWS, KJ
通讯作者：
MATTHEWS, KJ
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
氮营养状况导致云南杨对镉的反应存在性别差异
影响因子：
6.9
作者：
Chen L;Han Y;Jiang H;Korpelainen H;Li C
通讯作者：
Li C
DOI：
10.1016/s1043-4526(08)00401-4
发表时间：
2009-01-01
期刊：
ADVANCES IN FOOD AND NUTRITION RESEARCH, VOL 55
影响因子：
--
作者：
Christensen, Lars P.
通讯作者：
Christensen, Lars P.
影响因子：
4.2
作者：
Dzafic, Edo;Pongrac, Paula;Vogel-Mikus, Katarina
通讯作者：
Vogel-Mikus, Katarina