Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12
Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12
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大肠杆菌 K-12 中编码血红蛋白样蛋白的 hmp 基因的分离和核苷酸序列
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
R. Poole
中科院分区：
文献类型：
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作者：
S. Vasudevan;W. Armarego;Denis C. Shawl;P. E. Lilley;N. Dixon;R. Poole
在试图鉴定编码大肠杆菌二氢蝶啶还原酶（DHPR）活性的基因的过程中，分离了指导Mr 44000和46000的两种可溶性多肽合成的染色体DNA片段。这些蛋白质被部分纯化，并确定其N-末端氨基酸序列。较大的是丝氨酸羟甲基转移酶，由glyA基因编码，而较小的是先前描述的与glyA紧密相连的未命名基因的产物，并以相反的方向转录。E.过量产生44 kDa蛋白质的大肠杆菌细胞具有升高的DHPR活性，并且颜色为黄色。它们的可见吸收光谱表明CO结合的b型血蛋白在还原状态下是高自旋的。从该基因的完整核苷酸序列推导出的蛋白质的N-末端139个残基的序列与几乎所有的玻璃球菌血红蛋白具有广泛的同源性。我们的结论是E.大肠杆菌产生一种可溶性血红蛋白样蛋白，即hmp基因的产物（用于血红蛋白）。虽然该蛋白具有DHPR活性，但它与先前纯化的E. coli DHPR。
SummaryIn the course of an attempt to identify genes that encode Escherichia coli dihydropteridine reductase (DHPR) activities, a chromosomal DNA fragment that directs synthesis of two soluble polypeptides of Mr 44000 and 46000 was isolated. These proteins were partially purified and were identified by determination of their N-terminal amino acid sequences. The larger was serine hydroxymethyltransferase, encoded by the glyA gene, while the smaller was the previously described product of an unnamed gene closely linked to glyA, and transcribed in the opposite direction. Soluble extracts of E. coli cells that overproduced the 44 kDa protein had elevated DHPR activity, and were yellow in colour. Their visible absorption spectra were indicative of a CO-binding b-type haemoprotein that is high-spin in the reduced state. The sequence of the N-terminal 139 residues of the protein, deduced from the complete nucleotide sequence of the gene, had extensive homology to almost all of Vitreoscilla haemoglobin. We conclude that E. coli produces a soluble haemoglobin-like protein, the product of the hmp gene (for haemoprotein). Although the protein has DHPR activity, it is distinct from the previously purified E. coli DHPR.
影响因子：
3.5
作者：
STAUFFER, GV;PLAMANN, MD;STAUFFER, LT
通讯作者：
STAUFFER, LT
大肠杆菌 glyA 基因的完整核苷酸序列。
影响因子：
14.9
作者：
Plamann,MD;Stauffer,LT;Urbanowski,ML;Stauffer,GV
通讯作者：
Stauffer,GV