New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.
New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.
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DOI：
10.1093/plphys/kiac373
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发表时间：
2022-10-27
期刊：
影响因子：
7.4
通讯作者：
McCormick, Alistair J.
中科院分区：
文献类型：
--
作者：
Adler, Liat;Diaz-Ramos, Aranzazu;Mao, Yuwei;Pukacz, Krzysztof Robin;Fei, Chenyi;McCormick, Alistair J.
许多光合植物都进化出了二氧化碳浓缩机制（CCMs），以提高Rubisco对二氧化碳的同化效率，减少光呼吸的负面影响。然而，大多数植物（即C3植物）缺乏活性CCM。因此，将功能性异源CCM植入重要的C3作物，如水稻（Oryza sativa）和小麦（Triticum aestivum），已成为提高产量潜力的关键战略目标。本文综述了近年来对模型绿藻莱茵衣藻（Chlamydomonas reinhardtii）中pyrenoids基CCM的研究进展以及C3植物的工程进展。我们还讨论了最近的建模工作，这些工作提供了对类pyrenoid内Rubisco缩合的潜在优势和衣藻CCM的能量成本的见解，这些工作将有助于更好地指导未来的工程方法。主要发现包括Rubisco缩合对羧基化效率的潜在好处，以及在类pyrenoid基质周围需要扩散屏障。我们讨论了CCM功能的最小组成部分，并且活性碳酸氢盐进口到叶绿体基质中可能不是植物中基于pyrenoid的CCM功能所必需的。因此，在新的挑战和机遇下，构建以pyrenoid为基础的CCM进入植物叶绿体以提高光合作用效率的路线图现在变得更加清晰。对类芘形成的研究已经导致了C3陆地植物中藻类二氧化碳浓缩机制的关键进展，最近的一个模型预测了未来工作的优化途径。
Many photosynthetic species have evolved CO2-concentrating mechanisms (CCMs) to improve the efficiency of CO2 assimilation by Rubisco and reduce the negative impacts of photorespiration. However, the majority of plants (i.e. C3 plants) lack an active CCM. Thus, engineering a functional heterologous CCM into important C3 crops, such as rice (Oryza sativa) and wheat (Triticum aestivum), has become a key strategic ambition to enhance yield potential. Here, we review recent advances in our understanding of the pyrenoid-based CCM in the model green alga Chlamydomonas reinhardtii and engineering progress in C3 plants. We also discuss recent modeling work that has provided insights into the potential advantages of Rubisco condensation within the pyrenoid and the energetic costs of the Chlamydomonas CCM, which, together, will help to better guide future engineering approaches. Key findings include the potential benefits of Rubisco condensation for carboxylation efficiency and the need for a diffusional barrier around the pyrenoid matrix. We discuss a minimal set of components for the CCM to function and that active bicarbonate import into the chloroplast stroma may not be necessary for a functional pyrenoid-based CCM in planta. Thus, the roadmap for building a pyrenoid-based CCM into plant chloroplasts to enhance the efficiency of photosynthesis now appears clearer with new challenges and opportunities. Research on pyrenoid formation has led to key advances toward engineering an algal CO2-concentrating mechanism into C3 land plants, and a recent model predicts an optimized pathway for future work.
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影响因子：
11.6
作者：
Brueggeman, Andrew J.;Gangadharaiah, Dayananda S.;Ladunga, Istvan
通讯作者：
Ladunga, Istvan
SBPase活性增加可改善温室条件下生长的小麦的光合作用和谷物产量。
DOI：
10.1098/rstb.2016.0384
发表时间：
2017-09-26
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Driever SM;Simkin AJ;Alotaibi S;Fisk SJ;Madgwick PJ;Sparks CA;Jones HD;Lawson T;Parry MAJ;Raines CA
通讯作者：
Raines CA
影响因子：
7.4
作者：
Benlloch, Reyes;Shevela, Dmitriy;Sauer-Eriksson, A. Elisabeth
通讯作者：
Sauer-Eriksson, A. Elisabeth
影响因子：
7.4
作者：
COLEMAN, JR;BERRY, JA;GROSSMAN, AR
通讯作者：
GROSSMAN, AR
影响因子：
64.8
作者：
Burlacot, Adrien;Dao, Ousmane;Peltier, Gilles
通讯作者：
Peltier, Gilles