Effects of maternal obesity in an ovine model on metabolic outcomes in F2 adults and F3 neonates.
Effects of maternal obesity in an ovine model on metabolic outcomes in F2 adults and F3 neonates.
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DOI：
10.1016/j.domaniend.2021.106628
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发表时间：
2021-07
影响因子：
2.1
通讯作者：
Ford SP
中科院分区：
文献类型：
--
作者：
Pankey CL;Odhiambo JF;Smith AM;Ford SP
越来越多的证据表明，代谢综合征的迹象可以通过生殖系遗传，作为母亲肥胖的结果。我们假设，妊娠期间饮食引起的母亲肥胖会对后代多代产生代谢影响，即使第一代、第二代和第三代后代（分别为F1、F2、F3）只按要求喂养。对照（CON）和肥胖（OB）母羊（第0代，第0代）与一只公羊杂交，产生第一代后代（F1）。从受孕前60天到足月，con0母羊100%食用美国国家研究委员会建议（NRC），而OBF0母羊食用150% NRC。断奶后F1、F2和F3均饲喂100% NRC。将所有成熟F1母羊与一只公羊杂交，产生CONF2 （n=6）和OBF2 （n=10）。将所有成熟F2母羊与一只公羊杂交，生产CONF3 （n=6）和OBF3 （n=10）。妊娠期间，OBF2母羊血浆皮质醇水平高于CONF2母羊（P<0.0001）。妊娠90%时的葡萄糖耐量试验显示，在相同的葡萄糖条件下，OBF2母羊的胰岛素反应更高（P<0.05），导致更大的胰岛素抵抗（P<0.05）。OBF3新生儿的体重、瘦质量和体脂质量与CONF3新生儿相似。这些数据表明，不良代谢表型的多代编程与F0母亲肥胖有关，但肥胖可能在F3新生儿中恢复到CON水平。
Accumulating evidence suggests that indications of metabolic syndrome can be inherited through the germline as a result of maternal obesity. We hypothesized that diet-induced maternal obesity during gestation would program metabolic consequences for multiple generations of offspring, even when first, second, and third generation offspring (F1, F2, F3, respectively) were fed only to requirements. Control (CON) and obese (OB) ewes (generation 0; F0) were bred to a single ram to produce the first generation of offspring (F1). From 60 d prior to conception through term, CONF0 ate 100% National Research Council recommendations (NRC), while OBF0 ewes ate 150% NRC. All F1, F2, and F3 ate 100% NRC after weaning. All mature F1 ewes were bred to a single ram to generate CONF2 (n=6) and OBF2 (n=10). All mature F2 ewes were bred to a single ram to produce CONF3 (n=6) and OBF3 (n=10). OBF2 ewes exhibited greater (P<0.0001) plasma cortisol than CONF2 throughout gestation. A glucose tolerance test at 90% gestation revealed OBF2 ewes had higher (P<0.05) insulin response with similar glucose, resulting in greater (p<0.05) insulin resistance. OBF3 neonates had similar weight, lean mass, and body fat mass to CONF3 neonates. These data suggest that multigenerational programming of adverse metabolic phenotypes occur in association with F0 maternal obesity, yet adiposity may return to CON levels in F3 neonates.
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影响因子：
7.3
作者：
Fernandez-Twinn DS;Constância M;Ozanne SE
通讯作者：
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