Evaluation of ghost cell survival in the area of radiofrequency ablation.
Evaluation of ghost cell survival in the area of radiofrequency ablation.
复制标题
射频消融区域鬼细胞存活率的评估
DOI：
10.1371/journal.pone.0053158
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Wang Q;Huang J;Ma K;Li T;Chen M;Wang S;Bie P;He Z
研究人员已经证明，射频消融（RFA）病变中的死细胞与活的肿瘤细胞具有形态学上的相似性，因此被称为鬼细胞。然而，关于幽灵细胞持续多久的研究还没有系统地进行。将VX2肿瘤组织植入48只新西兰兔肝脏，建立肿瘤模型。两周后，RFA清除了这些肿瘤。在治疗后0周、1周、2周、4周、8周或12周切除病变，并用苏木精和伊红（HE）或烟酰胺腺嘌呤二核苷酸（NADH）染色。用光学显微镜观察细胞的存在和它们所经历的形态变化。RFA 4周后，he染色的鬼影细胞未见明显形态学改变，NADH染色未见活细胞。RFA 8周后，细胞结构变得模糊。RFA 12周后，鬼影细胞不复存在。鬼影细胞的形态特征保持至少4周，在此期间，HE染色不能用于区分鬼影细胞和残余肿瘤细胞。细胞活力的NADH染色是区分残余肿瘤细胞和幽灵细胞的必要条件。这一证据增加了我们对RFA治疗实体瘤的机制的理解。
Researchers have demonstrated dead cells in radiofrequency ablation (RFA) lesions that have morphological similarities to viable tumor cells and are thus referred to as ghost cells. However, studies on how long ghost cells persist have not been systematically performed. A tumor model was established by implanting VX2 tumor tissue into the livers of 48 New Zealand rabbits. Two weeks later, these tumors were eliminated with RFA. The lesions were resected at 0 weeks, 1 week, 2 weeks, 4 weeks, 8 weeks, or 12 weeks after treatment, and samples were stained either with hematoxylin and eosin (HE) or nicotinamide adenine dinucleotide (NADH). The presence of the cells and the morphological changes that they underwent were examined by light microscopy. Four weeks after RFA, there were no obvious morphological changes observed in HE-stained ghost cells, and NADH staining revealed no viable cells. Eight weeks after RFA, the cell structure became indistinct. Twelve weeks after RFA, ghost cells were no longer present. The morphological characteristics of ghost cells are maintained for at least 4 weeks, during which time HE staining cannot be used to differentiate ghost cells from residual tumor cells. NADH staining for cell viability is necessary to differentiate residual tumor cells from ghost cells. This evidence adds to our understanding of the mechanisms of RFA when used on solid tumors.
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影响因子：
4.8
作者：
Kim SK;Lim HK;Ryu JA;Choi D;Lee WJ;Lee JY;Lee JH;Sung YM;Cho EY;Hong SM;Kim JS
通讯作者：
Kim JS
DOI：
10.1007/s00464-001-0006-6
发表时间：
2001-11-01
影响因子：
3.1
作者：
Isbert, C;Roggan, A;Germer, CT
通讯作者：
Germer, CT
DOI：
10.1016/s0748-7983(03)00146-x
发表时间：
2003-11-01
期刊：
EUROPEAN JOURNAL OF SURGICAL ONCOLOGY
影响因子：
--
作者：
Mutsaerts, ELAR;Van Coevorden, F;Van Gulik, TM
通讯作者：
Van Gulik, TM
影响因子：
9.6
作者：
Glantzounis, GK;Yang, W;Davidson, BR
通讯作者：
Davidson, BR
影响因子：
3.8
作者：
Mazzaglia, Peter J.;Berber, Eren;Siperstein, Aflan E.
通讯作者：
Siperstein, Aflan E.