The role and mechanism of miR-374 regulating the malignant transformation of mesenchymal stem cells.
The role and mechanism of miR-374 regulating the malignant transformation of mesenchymal stem cells.
复制标题
miR-374调控间充质干细胞恶性转化的作用及机制
DOI：
--
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发表时间：
2018
影响因子：
2.2
通讯作者：
Qian Hui
中科院分区：
文献类型：
--
作者：
Sun Zixuan;Chen Jingyan;Zhang Jiao;Ji Runbi;Xu Wenrong;Zhang Xu;Qian Hui
MicroRNAs (miRNAs) play important roles in cell transformation and carcinogenesis. We have previously established a tumor cell line K3 transformed from rat bone marrow-derived mesenchymal stem cells (rBM-MSCs). However, the underlying mechanism involved in MSC transformation remains unclear. Herein, we identified the key miRNAs that regulate the transformation of rBM-MSCs, and clarified their biological roles. Microarray and qRT-PCR results showed an increased expression of miR-374 but decreased expressions of miR-199a, miR-145, miR-34a, and miR-214 in K3 cells compared to rBM-MSCs. MiR-374 overexpression in rBM-MSCs increased the colony number and the proportion of the cells in S-phase. In addition, miR-374 overexpression reduced E-cadherin expression and increased N-cadherin expression in rBM-MSCs, promoting the migration ability of these cells. On the contrary, miR-374 knockdown in K3 cells led to impaired proliferation and migration capacities. Furthermore, wnt5a was identified as a target gene of miR-374. MiR-374 overexpression upregulated β-catenin expression in rBM-MSCs while miR-374 knockdown downregulated that in K3 cells. In conclusion, miR-374 promotes the proliferation and migration of transformed MSCs by regulating Wnt5a/β-catenin signaling pathway, which provides evidence for the contribution of miRNA to MSC transformation and suggests a new role of miR-374 in cancer development and progression.
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影响因子：
6.4
作者：
Mele, Valentina;Muraro, Manuele G.;Calabrese, Diego;Pfaff, Dennis;Amatruda, Nunzia;Amicarella, Francesca;Kvinlaug, Brynn;Bocelli-Tyndall, Chiara;Martin, Ivan;Resink, Therese J.;Heberer, Michael;Oertli, Daniel;Terracciano, Luigi;Spagnoli, Giulio C.;Iezzi, Giandomenica
通讯作者：
Iezzi, Giandomenica
体外自发转化后间充质干细胞中p53和survivin基因之间相互作用的丧失
DOI：
10.1016/j.biocel.2016.03.018
发表时间：
2016
影响因子：
4
作者：
He Liu;Zhao Fangyu;Zheng Yong;Wan Yu;Song Jian
通讯作者：
Song Jian
影响因子：
4.7
作者：
Xu, Xuejing;Qian, Hui;Xu, Wenrong
通讯作者：
Xu, Wenrong
影响因子：
8
作者：
Han B;Zhou B;Qu Y;Gao B;Xu Y;Chung S;Tanaka H;Yang W;Giuliano AE;Cui X
通讯作者：
Cui X
影响因子：
5
作者：
Zhou K;Liu M;Cao Y
通讯作者：
Cao Y