Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat.
Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat.
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DOI：
10.1016/j.neuroscience.2009.12.053
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发表时间：
2010-03-17
期刊：
影响因子：
3.3
通讯作者：
Rance, N. E.
中科院分区：
文献类型：
--
作者：
Krajewski, S. J.;Burke, M. C.;Anderson, M. J.;McMullen, N. T.;Rance, N. E.
神经激肽B （NKB）和kisspeptin受体信号是生殖轴的重要组成部分。在大鼠的弓形核内有一群神经元表达NKB、kisspeptin、dynorphin、NK3受体和雌激素受体α。在这里，我们使用nkb免疫细胞化学作为标记来研究这些神经元的投影。首先，新生儿注射谷氨酸钠消融弓形核后，nkb免疫反应（ir）体细胞和纤维的损失被表征。其次，将生物素化的葡聚糖胺注射到弓形核中，用双标记免疫荧光法鉴定顺行标记的NKB-ir纤维。描述了四种主要的投射路径：1)两侧弓形核内的局部投射，2)向中隆起（包括外侧栅栏区）的投射，3)向室周路径的投射，向侧延伸至多个下丘脑核、间隔区和BNST，向背延伸至背内侧核，4)向下丘脑腹侧束的投射，向外侧下丘脑和内侧前脑束。这些不同的投射表明，NKB/kisspeptin/dynorphin神经元可能将生殖轴与多种稳态、行为和神经内分泌过程整合在一起。有趣的是，顺行束示迹显示，NKB-ir轴突起源于弓形神经元，终止于弓形核内的其他NKB-ir体细胞。结合以往的研究，这些实验揭示了大鼠弓形核中一个双侧相互连接的性类固醇反应神经元网络，表达NKB、kisspeptin、dynorphin、NK3受体和ERα，并投射到正中隆起的GnRH末端。该回路提供了弓形NKB/kisspeptin/dynorphin神经元双侧同步调节GnRH脉动分泌的机制。
Neurokinin B (NKB) and kisspeptin receptor signaling are essential components of the reproductive axis. A population of neurons resides within the arcuate nucleus of the rat that expresses NKB, kisspeptin, dynorphin, NK3 receptors and estrogen receptor α. Here we investigate the projections of these neurons using NKB-immunocytochemistry as a marker. First, the loss of NKB-immunoreactive (ir) somata and fibers was characterized after ablation of the arcuate nucleus by neonatal injections of monosodium glutamate. Second, biotinylated dextran amine was injected into the arcuate nucleus and anterogradely labeled NKB-ir fibers were identified using dual-labeled immunofluorescence. Four major projection pathways are described: 1) Local projections within the arcuate nucleus bilaterally, 2) Projections to the median eminence including the lateral palisade zone, 3) Projections to a periventricular pathway extending rostrally to multiple hypothalamic nuclei, the septal region and BNST and dorsally to the dorsomedial nucleus and 4) Projections to a ventral hypothalamic tract to the lateral hypothalamus and medial forebrain bundle. The diverse projections provide evidence that NKB/kisspeptin/dynorphin neurons could integrate the reproductive axis with multiple homeostatic, behavioral and neuroendocrine processes. Interestingly, anterograde tract-tracing revealed NKB-ir axons originating from arcuate neurons terminating on other NKB-ir somata within the arcuate nucleus. Combined with previous studies, these experiments reveal a bilateral interconnected network of sex-steroid responsive neurons in the arcuate nucleus of the rat that express NKB, kisspeptin, dynorphin, NK3 receptors and ERα and project to GnRH terminals in the median eminence. This circuitry provides a mechanism for bilateral synchronization of arcuate NKB/kisspeptin/dynorphin neurons to modulate the pulsatile secretion of GnRH.
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影响因子：
--
作者：
Couse, JF;Yates, MM;Korach, KS
通讯作者：
Korach, KS
影响因子：
56.9
作者：
BELCHETZ, PE;PLANT, TM;KNOBIL, E
通讯作者：
KNOBIL, E
DOI：
10.1073/pnas.1834399100
发表时间：
2003-09-16
影响因子：
11.1
作者：
de Roux, N;Genin, E;Milgrom, E
通讯作者：
Milgrom, E
影响因子：
4.8
作者：
CLEMENS, JA;ROUSH, ME;SHAAR, CJ
通讯作者：
SHAAR, CJ
影响因子：
4.8
作者：
Dellovade, TL;Merchenthaler, I
通讯作者：
Merchenthaler, I