Transcriptional profiling of mouse cavernous pericytes under high-glucose conditions: Implications for diabetic angiopathy.
Transcriptional profiling of mouse cavernous pericytes under high-glucose conditions: Implications for diabetic angiopathy.
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高血糖条件下小鼠海绵体周细胞的转录谱：对糖尿病血管病的影响
DOI：
10.4111/icu.20200272
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发表时间：
2021-01
影响因子：
2.3
通讯作者：
Jin HR
中科院分区：
文献类型：
--
作者：
Yin GN;Wu J;Cui Y;Lin C;Shi L;Gao ZL;Suh JK;Ryu JK;Jin HR
目的阴茎勃起需要血管内皮细胞、周细胞、平滑肌细胞和自主神经之间的整合作用。此外，周细胞在血管病发病机制中所起作用的重要性最近才被认识到。然而，糖尿病性勃起功能障碍（DMED）中周细胞的整体基因表达仍不清楚。我们的目的是确定潜在的靶基因DMED在小鼠海绵状周细胞（MCP）。材料与方法将小鼠海绵体组织置于I型胶原包被的培养皿中，在重力作用下沉降，传代培养发芽细胞用于实验。为了模拟糖尿病状况，将MCP用正常葡萄糖（NG，5 mM）或高葡萄糖（HG，30 mM）培养基处理3天。基因芯片技术用于评估基因表达谱，RT-PCR用于验证测序数据。采用组织学检查和Western blot对最终筛选的DMED相关靶基因进行验证。结果高糖组MCP细胞微管形成减少，凋亡增加。如微阵列分析所示，暴露于NG或HG条件的MCP的基因表达谱不同。共有2，523个表达显著改变的基因被分为15个主要基因类别。进一步的基因表达和RT-PCR及组织学结果的基础上筛选，我们发现，Hebp 1基因的表达显着降低HG条件下和DM小鼠。结论这项基因谱研究为MCP中糖尿病的发生提供了新的潜在靶点。验证研究表明，Hebp 1可能是DMED的合适生物标志物。
Purpose Penile erection requires integrative interactions between vascular endothelial cells, pericytes, smooth muscle cells, and autonomic nerves. Furthermore, the importance of the role played by pericytes in the pathogenesis of angiopathy has only recently been appreciated. However, global gene expression in pericytes in diabetes mellitus-induced erectile dysfunction (DMED) remains unclear. We aimed to identify potential target genes related to DMED in mouse cavernous pericytes (MCPs). Materials and Methods Mouse cavernous tissue was allowed to settle under gravity in collagen I-coated dishes, and sprouted cells were subcultivated for experiments. To imitate diabetic conditions, MCPs were treated with normal-glucose (NG, 5 mM) or high-glucose (HG, 30 mM) media for 3 days. Microarray technology was used to evaluate gene expression profiles, and RT-PCR was used to validate sequencing data. Histological examinations and Western blot were used to validate final selected target genes related to DMED. Results Decreased tube formation and increased apoptosis were detected in MCPs exposed to the HG condition. As shown by microarray analysis, the gene expression profiles of MCPs exposed to the NG or HG condition differed. A total of 2,523 genes with significantly altered expression were classified into 15 major gene categories. After further screening based on gene expression and RT-PCR and histologic results, we found that Hebp1 gene expression was significantly diminished under the HG condition and in DM mice. Conclusions This gene profiling study provides new potential targets responsible for diabetes in MCPs. Validation studies suggest that Hebp1 may be a suitable biomarker for DMED.
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影响因子：
11.2
作者：
Bagley, RG;Weber, W;Teicher, BA
通讯作者：
Teicher, BA
影响因子：
7.7
作者：
Detaille, D;Guigas, B;Leverve, X
通讯作者：
Leverve, X
影响因子：
25
作者：
Winkler EA;Bell RD;Zlokovic BV
通讯作者：
Zlokovic BV
影响因子：
14.8
作者：
通讯作者：
--
影响因子：
20.3
作者：
Bussolati, B;Ahmed, A;Mason, JC
通讯作者：
Mason, JC