Zinc sparks are triggered by fertilization and facilitate cell cycle resumption in mammalian eggs.
Zinc sparks are triggered by fertilization and facilitate cell cycle resumption in mammalian eggs.
复制标题
DOI：
10.1021/cb200084y
复制
发表时间：
2011-07-15
影响因子：
4
通讯作者：
O'Halloran, Thomas V.
中科院分区：
文献类型：
--
作者：
Kim, Alison M.;Bernhardt, Miranda L.;Kong, Betty Y.;Ahn, Richard W.;Vogt, Stefan;Woodruff, Teresa K.;O'Halloran, Thomas V.
在成熟的最后几个小时，小鼠卵母细胞吸收超过200亿个锌原子，并在第一次减数分裂后停止，直到受精或药物干预刺激细胞周期向新胚胎发展。使用化学和物理探针，我们表明，受精的成熟，锌丰富的鸡蛋触发锌喷射到细胞外环境的一系列协调事件称为锌火花。这些事件紧随激活卵子内钙振荡的既定序列，并在包括啮齿类动物和非人灵长类动物在内的几种哺乳动物物种中具有进化保守性。在功能上，锌火花介导细胞内锌含量的减少，这是细胞周期持续进展所必需的，因为激活卵子内锌水平的增加导致细胞周期中期停滞的重建。哺乳动物卵子因此使用锌依赖的开关机制，在胚胎发育开始时在中期停止和减数分裂细胞周期的恢复之间切换。
In last few hours of maturation, the mouse oocyte takes up over twenty billion zinc atoms and arrests after the first meiotic division, until fertilization or pharmacological intervention stimulates cell cycle progression towards a new embryo. Using chemical and physical probes, we show that fertilization of the mature, zinc-enriched egg triggers the ejection of zinc into the extracellular milieu in a series of coordinated events termed zinc sparks. These events immediately follow the well-established series of calcium oscillations within the activated egg and are evolutionarily conserved in several mammalian species, including rodents and non-human primates. Functionally, the zinc sparks mediate a decrease in intracellular zinc content that is necessary for continued cell cycle progression, as increasing zinc levels within the activated egg results in the reestablishment of cell cycle arrest at metaphase. The mammalian egg thus uses a zinc-dependent switch mechanism to toggle between metaphase arrest and resumption of the meiotic cell cycle at the initiation of embryonic development.
登录
查看更多内容
影响因子：
2.7
作者：
LONGO, FJ;CHEN, DY
通讯作者：
CHEN, DY
影响因子：
9.2
作者：
Liu, JJ;Maller, JL
通讯作者：
Maller, JL
影响因子：
2.7
作者：
Markoulaki, S;Matson, S;Ducibella, T
通讯作者：
Ducibella, T
影响因子：
2.7
作者：
Ducibella, T;Huneau, D;Ozil, JP
通讯作者：
Ozil, JP
影响因子：
2.7
作者：
Duncan, FE;Moss, SB;Williams, CJ
通讯作者：
Williams, CJ