Bioengineering the Bone Marrow Vascular Niche.
Bioengineering the Bone Marrow Vascular Niche.
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DOI：
10.3389/fcell.2021.645496
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发表时间：
2021
影响因子：
5.5
通讯作者：
Passaro D
中科院分区：
文献类型：
--
作者：
Bessy T;Itkin T;Passaro D
骨髓组织是成人造血的主要生理部位。近年来，构成BM的细胞和基质组分已经在分子和结构水平上以前所未有的分辨率被定义。随着知识的扩展，复制BM样结构以异位支持和研究造血的可能性成为现实。已经实施了许多实验系统，并且已经显示了由间充质来源的细胞支持的生物工程BM组织的可行性。尽管被认为是BM的丰富成分，但脉管系统在调节组织形成、组织和决定中的作用在很大程度上被忽视。最近的报道强调了血管内皮细胞在产前和产后形成组织发育和支持稳态、紧急和恶性造血中的关键作用。在此，我们回顾了BM-组织生物工程领域，特别关注血管系统的实施和整合，从描述各种适用的体外模型开始，以体内临床前模型结束。此外，我们强调了该领域的挑战，并讨论了在患者中过继转移血管化BM-龛移植物以支持恢复造血的临床前景。
The bone marrow (BM) tissue is the main physiological site for adult hematopoiesis. In recent years, the cellular and matrix components composing the BM have been defined with unprecedent resolution, both at the molecular and structural levels. With the expansion of this knowledge, the possibility of reproducing a BM-like structure, to ectopically support and study hematopoiesis, becomes a reality. A number of experimental systems have been implemented and have displayed the feasibility of bioengineering BM tissues, supported by cells of mesenchymal origin. Despite being known as an abundant component of the BM, the vasculature has been largely disregarded for its role in regulating tissue formation, organization and determination. Recent reports have highlighted the crucial role for vascular endothelial cells in shaping tissue development and supporting steady state, emergency and malignant hematopoiesis, both pre- and postnatally. Herein, we review the field of BM-tissue bioengineering with a particular focus on vascular system implementation and integration, starting from describing a variety of applicable in vitro models, ending up with in vivo preclinical models. Additionally, we highlight the challenges of the field and discuss the clinical perspectives in terms of adoptive transfer of vascularized BM-niche grafts in patients to support recovering hematopoiesis.
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23.9
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2.3
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通讯作者：
Rodriguez-Pardo, Viviana M.