Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues.
Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues.
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DOI：
10.1186/gb-2004-5-3-r16
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发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Walbot V
中科院分区：
文献类型：
--
作者：
Casati P;Walbot V
分析玉米组织对UV-B辐射的转录反应表明，信号从受辐射的组织传递到屏蔽的组织。转录反应发生迅速，即使在屏蔽组织。平流层臭氧的消耗提高了地面的紫外线-B辐射水平，这是一种与皮肤癌风险增加有关的环境变化，并对植物产生潜在的有害后果。为了更好地了解UV-B驯化过程中，改变植物形态和生理，我们研究了基因表达在不同器官的玉米在几个UV-B的能量密度和曝光时间。微阵列杂交被用来评估UV-B直接暴露的玉米器官和器官屏蔽吸收UV-B的塑料。经过8个小时的高UV-B，347个转录本的丰度被改变：285个在至少一个器官中显著增加，80个下调。直接暴露的器官中转录本的变化比屏蔽的器官中多，并且成年组织中转录本的水平比幼苗组织中多。转录丰度变化的时间过程表明，UV-B的响应动力学是非常迅速的，因为一些转录水平在1小时内的曝光改变。大多数UV-B调控基因是器官特异性的。由于被屏蔽的组织，包括根，未成熟的耳朵，和叶子，显示改变的转录谱后，植物暴露于UV-B，一些信号（S）必须从辐射传输到屏蔽的组织。这些结果表明，有超过正常水平的UV-B辐射的综合反应。由于相同的总UV-B照射剂量在三个强度引起不同的转录谱，转录组的变化表现出阈值效应，而不是相互的剂量效应反应。转录组分析突出了未来研究的可能信号通路和分子。
Profiling the transcriptional response of maize tissues to UV-B irradiation suggests that a signal is transmitted from irradiated to shielded tissue. The transcriptional response occurs rapidly, even in shielded tissue. Depletion of stratospheric ozone has raised terrestrial levels of ultraviolet-B radiation (UV-B), an environmental change linked to an increased risk of skin cancer and with potentially deleterious consequences for plants. To better understand the processes of UV-B acclimation that result in altered plant morphology and physiology, we investigated gene expression in different organs of maize at several UV-B fluence rates and exposure times. Microarray hybridization was used to assess UV-B responses in directly exposed maize organs and organs shielded by a plastic that absorbs UV-B. After 8 hours of high UV-B, the abundance of 347 transcripts was altered: 285 were increased significantly in at least one organ and 80 were downregulated. More transcript changes occurred in directly exposed than in shielded organs, and the levels of more transcripts were changed in adult compared to seedling tissues. The time course of transcript abundance changes indicated that the response kinetics to UV-B is very rapid, as some transcript levels were altered within 1 hour of exposure. Most of the UV-B regulated genes are organ-specific. Because shielded tissues, including roots, immature ears, and leaves, displayed altered transcriptome profiles after exposure of the plant to UV-B, some signal(s) must be transmitted from irradiated to shielded tissues. These results indicate that there are integrated responses to UV-B radiation above normal levels. As the same total UV-B irradiation dose applied at three intensities elicited different transcript profiles, the transcriptome changes exhibit threshold effects rather than a reciprocal dose-effect response. Transcriptome profiling highlights possible signaling pathways and molecules for future research.
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影响因子：
11.4
作者：
Hishida, T;Ohno, T;Shinagawa, H
通讯作者：
Shinagawa, H
影响因子：
7.4
作者：
Holley, SR;Yalamanchili, RD;Stratmann, JW
通讯作者：
Stratmann, JW
DOI：
10.1016/s1011-1344(01)00152-x
发表时间：
2001-09-01
影响因子：
5.4
作者：
Ballaré, CL;Rousseaux, MC;Scopel, AL
通讯作者：
Scopel, AL
影响因子：
11.6
作者：
Christie, JM;Jenkins, GI
通讯作者：
Jenkins, GI
影响因子：
64.8
作者：
Bao, SD;Tibbetts, RS;Wang, XF
通讯作者：
Wang, XF