Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.
Characterization of novel microneme adhesive repeats (MAR) in Eimeria tenella.
复制标题
DOI：
10.1186/s13071-017-2454-4
复制
发表时间：
2017-10-17
影响因子：
3.2
通讯作者：
Tomley FM
中科院分区：
文献类型：
--
作者：
Marugan-Hernandez V;Fiddy R;Nurse-Francis J;Smith O;Pritchard L;Tomley FM
顶复门包括多种具有重要医学和经济意义的寄生虫。这些寄生虫具有极其不同的宿主和组织嗜性;例如，弓形虫几乎可以侵入任何有核细胞并感染几乎所有的温血脊椎动物，而柔嫩艾美耳球虫仅感染鸡，并且仅限于盲肠的上皮细胞生长。从微丝分泌细胞器（MIC）释放的蛋白质是顶复体入侵宿主细胞的关键，并允许寄生虫结合多种宿主细胞寡糖表位。MIC具有粘附蛋白序列的模块化排列，有趣的是，含有微线粘附重复序列（microneme adhesive repeat，MAR）结构域的蛋白（MCP）被认为对T. gondii和E. tenella。在这项研究中，我们评估了新的大肠杆菌I型MAR结构域的结合能力。tenella MCP。对先前描述的HxT基序的变体进行分析，显示HxT和YxT变体结合，而HxS和YxE变体不结合。其中一个MCP含有一个单一的MAR（EtMCP 2）表现出顶端定位时，表达为与荧光报告mCherry在转基因群体中的融合和类似的模式，转录每个子体在体外内源性发育过程中，作为良好表征的微线体蛋白EtMIC 2。证实了不同EtMCP的MAR结合特性的变化，应研究其结合更广泛唾液酸和末端连接的能力。此外，转基因技术已首次在艾美耳球虫寄生虫中作为一种快速的工具，通过与荧光报告基因的融合来研究内源性蛋白质定位。本文的在线版本（10.1186/s13071-017-2454-4）包含补充材料，可供授权用户使用。
The phylum Apicomplexa comprises a wide variety of parasites of significant medical and economic relevance. These parasites have extremely different host and tissue tropisms; for example Toxoplasma gondii can invade virtually any nucleated cell and infect almost all warm-blooded vertebrates, whereas Eimeria tenella infects only chickens and is restricted in its growth to epithelial cells of the caecum. Proteins released from the microneme secretory organelles (MICs) are critical for apicomplexan invasion of host cells and allow parasites to bind a diverse range of host cell oligosaccharide epitopes. MICs bear modular arrangements of sequences with adhesive proteins and interestingly the sialic-acid binding MAR (microneme adhesive repeat) domain containing proteins (MCPs) are suggested to make significant contributions to the different host and tissue tropisms of T. gondii and E. tenella. In this study, we evaluated the binding capacity of Type I MAR domains from novel E. tenella MCPs. Variants of the previously described HxT motif were analysed showing that HxT and VxT variants bind, whereas HxS and YxE variants did not. One of these MCP containing a single MAR (EtMCP2) showed an apical localization when expressed as a fusion with the fluorescent reporter mCherry in transgenic populations and a similar pattern of transcripts per zoite during endogenous development in vitro as the well-characterised microneme protein EtMIC2. Variation in the binding properties of the MAR of different EtMCPs was confirmed and their ability to bind a wider range of sialic acids and terminal linkages should be studied. In addition, transgenesis technology has been used for first time in Eimeria parasites as a rapid tool for the study of endogenous protein localization by fusion with a fluorescent reporter. The online version of this article (10.1186/s13071-017-2454-4) contains supplementary material, which is available to authorized users.
登录
查看更多内容
ROP18是弓形虫与犬新孢子虫毒力差异的关键因素
影响因子：
3.7
作者：
Lei T;Wang H;Liu J;Nan H;Liu Q
通讯作者：
Liu Q
影响因子：
3.4
作者：
Lal, Kalpana;Bromley, Elizabeth;Oakes, Richard;Prieto, Judith Helena;Sanderson, Sanya J.;Kurian, Dominic;Hunt, Lawrence;Yates, John R., III;Wastling, Jonathan M.;Sinden, Robert E.;Tomley, Fiona M.
通讯作者：
Tomley, Fiona M.
影响因子：
6.4
作者：
Chen AL;Kim EW;Toh JY;Vashisht AA;Rashoff AQ;Van C;Huang AS;Moon AS;Bell HN;Bentolila LA;Wohlschlegel JA;Bradley PJ
通讯作者：
Bradley PJ
影响因子：
6.7
作者：
Lai L;Bumstead J;Liu Y;Garnett J;Campanero-Rhodes MA;Blake DP;Palma AS;Chai W;Ferguson DJ;Simpson P;Feizi T;Tomley FM;Matthews S
通讯作者：
Matthews S
影响因子：
4.6
作者：
Marugan-Hernandez V;Long E;Blake D;Crouch C;Tomley F
通讯作者：
Tomley F