Proteomic comparison of four Eimeria tenella life-cycle stages: unsporulated oocyst, sporulated oocyst, sporozoite and second-generation merozoite.
Proteomic comparison of four Eimeria tenella life-cycle stages: unsporulated oocyst, sporulated oocyst, sporozoite and second-generation merozoite.
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DOI：
10.1002/pmic.200900305
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发表时间：
2009-10
期刊：
影响因子：
3.4
通讯作者：
Tomley, Fiona M.
中科院分区：
文献类型：
--
作者：
Lal, Kalpana;Bromley, Elizabeth;Oakes, Richard;Prieto, Judith Helena;Sanderson, Sanya J.;Kurian, Dominic;Hunt, Lawrence;Yates, John R., III;Wastling, Jonathan M.;Sinden, Robert E.;Tomley, Fiona M.
我们报告的蛋白质组的四个生命周期阶段的顶复门寄生虫柔嫩艾美耳球虫。共鉴定出1868个蛋白，其中早期卵囊（未形成孢子）、晚期卵囊（形成孢子）、子孢子和第二代裂殖子中分别发现630、699、845和1532个蛋白。MudPIT鸟枪法鉴定了812个子孢子，1528个裂殖子和所有的卵囊蛋白，而2D凝胶蛋白质组学鉴定了230个子孢子和98个裂殖子蛋白。比较侵入阶段，我们发现移动连接组件RON 2在两者中，而AMA-1和RON 4仅在裂殖子中发现，AMA-2和RON 5仅在子孢子中发现，这表明阶段特异性移动连接蛋白。在早期卵囊到子孢子的发育过程中，可触体和大多数“滑体”蛋白贯穿始终，而微线和大多数棒状体蛋白仅在孢子形成后才被发现。定量分析表明，糖酵解和再生是所有阶段中检测到的最丰富的代谢群。在裂殖子中未检测到糖酵解的甘露醇循环“分支”，但在其他阶段中有很好的代表性。然而，在裂殖子中，我们发现更多的蛋白质与氧化磷酸化相关，这表明代谢转变动员了更大的能量生产。我们发现裂殖子中与转录、蛋白质合成和细胞周期相关的蛋白质比子孢子中丰富，这可能是裂殖子生殖过程中大量复制的残留蛋白。
We report the proteomes of four life cycle stages of the Apicomplexan parasite Eimeria tenella. A total of 1868 proteins were identified, with 630, 699, 845 and 1532 found in early oocysts (unsporulated), late oocysts (sporulated), sporozoites and second-generation merozoites, respectively. A MudPIT shotgun approach identified 812 sporozoite, 1528 merozoite and all of the oocyst proteins, whereas 2D gel proteomics identified 230 sporozoite and 98 merozoite proteins. Comparing the invasive stages, we find moving junction components RON2 in both, whilst AMA-1 and RON4 are found only in merozoites and AMA-2 and RON5 are only found in sporozoites, suggesting stage specific moving junction proteins. During early oocyst to sporozoite development, refractile body and most ‘glideosome’ proteins are found throughout, whilst microneme and most rhoptry proteins are only found after sporulation. Quantitative analysis indicates glycolysis and gluconeogenesis are the most abundant metabolic groups detected in all stages. The mannitol cycle ‘off shoot’ of glycolysis was not detected in merozoites but was well represented in the other stages. However, in merozoites we find more protein associated with oxidative phosphorylation, suggesting a metabolic shift mobilising greater energy production. We find a greater abundance of protein linked to transcription, protein synthesis and cell cycle in merozoites than in sporozoites, which may be residual protein from the preceding massive replication during schizogony.
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影响因子：
--
作者：
Alexander, David L.;Arastu-Kapur, Shirin;Boothroyd, John C.
通讯作者：
Boothroyd, John C.
影响因子：
2.4
作者：
KAWAZOE, U;TOMLEY, FM;FRAZIER, JA
通讯作者：
FRAZIER, JA
影响因子：
14.9
作者：
Käll L;Krogh A;Sonnhammer EL
通讯作者：
Sonnhammer EL
影响因子：
4
作者：
de Venevelles, Patrick;Chich, Jean Francois;Labbe, Marie
通讯作者：
Labbe, Marie
影响因子：
1.5
作者：
Bender, A;van Dooren, GG;Schneider, G
通讯作者：
Schneider, G