Presence of Segmented Filamentous Bacteria in Human Children and Its Potential Role in the Modulation of Human Gut Immunity.
Presence of Segmented Filamentous Bacteria in Human Children and Its Potential Role in the Modulation of Human Gut Immunity.
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DOI：
10.3389/fmicb.2018.01403
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发表时间：
2018
影响因子：
5.2
通讯作者：
Xiang C
中科院分区：
文献类型：
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作者：
Chen B;Chen H;Shu X;Yin Y;Li J;Qin J;Chen L;Peng K;Xu F;Gu W;Zhao H;Jiang L;Li L;Song J;Elitsur Y;Yu HD;Jiang M;Wang X;Xiang C
分节丝状细菌（SFB）是一种通过将一种特殊的固着结构锚定在各种动物肠壁上而生长的肠道生物。SFB与小鼠派伊尔集合淋巴结的相互作用促进免疫系统的出生后成熟。我们以前报道过，SFB在人类中的定殖主要发生在36个月大的时候，之后很难被检测到。在这项研究中，我们测量了人类儿童肠液中SFB的水平。通过qPCR发现SFB代表整个SFB阳性微生物群的一小部分（1011个总细菌中有105个SFB）。通过荧光原位杂交在肠液中观察到丝状分段形态的细菌，并通过扫描电子显微镜从肠道活检中观察到。SFB特异性DNA和肽片段也通过多重置换扩增PCR和质谱鉴定。SFB的存在与总分泌型免疫球蛋白A（sIgA）的滴度之间存在总体正相关，这在8-36个月年龄组的肠液中更明显。之后，SFB的数量下降与总sIgA的减少相关。末端回肠活检的RT-qPCR分析显示，在SFB阳性样品中诱导了Th 17途径基因的表达，而T和B细胞受体信号传导途径的标志物也上调。总的来说，这些数据表明SFB是微生物群中的一个罕见成员，并且可能在人类肠道免疫的发展中发挥重要作用。
Segmented filamentous bacteria (SFB) are commensal organisms that grow by anchoring a specialized holdfast structure to the intestinal walls of a variety of animals. Interaction of SFB with Peyer’s patches in mice promotes the post-natal maturation of the immune system. We previously reported that the colonization of SFB in humans mainly occurs by 36 months of age, and is difficult to be detected afterward. In this study, we measured the level of SFB in intestinal fluids of human children. SFB were found via qPCR to represent a small fraction of the whole SFB-positive microbiota (105 SFB in 1011 total bacteria). Bacteria with filamentous segmented morphology were observed in intestinal fluids via fluorescent in situ hybridization, and from gut biopsies via scanning electron microscopy. SFB-specific DNA and peptide fragments were also identified via multiple displacement amplification PCR and mass spectrometry. There was an overall positive correlation between the presence of SFB and the titer of total secretory immunoglobulin A (sIgA), which is more apparent in intestinal fluids of the age group of 8–36 months. Afterward there was a decline of SFB in numbers correlated with a reduction of total sIgA. RT-qPCR analysis of the terminal ileal biopsies revealed that the expression of Th17 pathway genes were induced in SFB-positive samples, while the markers of T and B cell receptor signaling pathways were also upregulated. Collectively, these data suggest that SFB is a rare member of microbiota, and may play an important role in the development of human gut immunity.
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影响因子：
64.5
作者：
Ivanov, Ivaylo I.;McKenzie, Brent S.;Littman, Dan R.
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
8
作者：
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