Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus.
Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus.
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施氏假单胞菌273基因组序列及胞外多糖EPS273生物合成位点的鉴定
DOI：
10.3390/md15070218
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发表时间：
2017-07-10
期刊：
影响因子：
5.4
通讯作者：
Sun C
中科院分区：
文献类型：
--
作者：
Wu S;Zheng R;Sha Z;Sun C
施氏假单胞菌273是一种产生胞外多糖273（EPS 273）的海洋细菌，对铜绿假单胞菌PAO 1具有高抗生物膜活性。在此，对斯氏疟原虫273的全基因组进行了测序，该基因组包含一个环状5.03 Mb染色体。通过对基因组的广泛分析，预测包含18个基因的遗传位点参与EPS 273的生物合成。为了证实这一预测，删除了遗传基因座内编码糖基转移酶的两个相邻基因（eps 273-H和eps 273-I）和编码酪氨酸蛋白激酶的一个基因（eps 273-O），并平行检查了EPS 273的生物合成。从突变体Δ eps 273-HI中纯化的EPS分子量与从野生型斯氏毕赤酵母273中纯化的EPS分子量明显不同，而从突变体Δ eps 273-O中几乎检测不到相应的EPS，这表明所提出的18个基因簇参与了EPS 273的生物合成。此外，突变株Δ eps 273-HI的生物膜形成时间比野生型提前，而突变株Δ eps 273-O几乎完全丧失生物膜形成能力。因此，EPS 273可能促进其产生菌施氏假单胞菌273的生物被膜形成，而抑制铜绿假单胞菌PAO 1的生物被膜形成。该研究有助于更好地了解EPS 273的生物合成，并揭示EPS 273的生物学功能及其产生菌施氏毕赤酵母273。
Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P. stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel. The molecular weight profile of EPS purified from the mutant Δeps273-HI was obviously different from that purified from wild-type P. stutzeri 273, while the corresponding EPS was hardly detected from the mutant Δeps273-O, which indicated the involvement of the proposed 18-gene cluster in the biosynthesis of EPS273. Moreover, the mutant Δeps273-HI had the biofilm formed earlier compared with the wild type, and the mutant Δeps273-O almost completely lost the ability of biofilm formation. Therefore, EPS273 might facilitate the biofilm formation for its producing strain P. stutzeri 273 while inhibiting the biofilm formation of P. aeruginosa PAO1. This study can contribute to better understanding of the biosynthesis of EPS273 and disclose the biological function of EPS273 for its producing strain P. stutzeri 273.
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