Comprehensive Epstein-Barr Virus Transcriptome by RNA-Sequencing in Angioimmunoblastic T Cell Lymphoma (AITL) and Other Lymphomas.
Comprehensive Epstein-Barr Virus Transcriptome by RNA-Sequencing in Angioimmunoblastic T Cell Lymphoma (AITL) and Other Lymphomas.
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血管免疫母细胞性T细胞淋巴瘤（AITL）和其他淋巴瘤中通过RNA测序的全面EB病毒转录组。
DOI：
10.3390/cancers13040610
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发表时间：
2021-02-04
期刊：
影响因子：
5.2
通讯作者：
Ranger-Rogez S
中科院分区：
文献类型：
--
作者：
Bayda N;Tilloy V;Chaunavel A;Bahri R;Halabi MA;Feuillard J;Jaccard A;Ranger-Rogez S
血管免疫母细胞T细胞淋巴瘤(AITL)可能是最常见的外周T细胞淋巴瘤。这种病理虽然罕见，但在欧洲比在其他地区更常见。这种淋巴瘤的预后很差。AITL与Epstein-Barr病毒(EBV)非常常见，尽管该病毒不常在肿瘤性T细胞中发现，但在邻近的B细胞中更常见。我们的目的是研究AITL中EBV的转录组与其他EBV相关淋巴瘤的转录组，并将结果与细胞系的结果进行比较。我们发现在AITL中Bam-HI A向右转录本(BART)的表达高于其他淋巴瘤，尤其是在细胞系中。BARTS可以参与肿瘤的发展。AITL中存在潜伏期IIc，表达BNLF2a和BCRF1基因，可能参与感染细胞的存活。这些结果支持EBV参与AITLS的发生。在超过80%的病例中，EB病毒(EBV)与血管免疫母细胞T细胞淋巴瘤(AITL)有关。很少有研究关注这种联系，目前还不清楚病毒在这种病理中扮演了什么角色。我们使用下一代测序技术(NGS)研究了14个AITL中的EBV转录组，并与21个其他淋巴瘤样本和11个细胞系进行了比较，其中包括4个淋巴母细胞系(LCLS)。使用捕获探针恢复病毒转录本，并对Illumina进行测序。BAM-HI A向右转录本(BART)是AITL中表达的潜伏期最长的转录本，提示它们可能在AITL的病理过程中起一定作用。因此，已经被描述为在癌细胞中高表达的BART，在AITL和其他淋巴瘤中也非常存在。它们在LCLS以外的其他细胞系中表达很低。AITL表现为潜伏期IIc，伴有BNLF2a基因表达。对于大多数AITL，编码人白介素10的同源蛋白VIL-10的BCRF1是另外表达的。这种共表达有助于免疫逃逸和感染细胞的存活。考虑到这些结果，可以假设EBV在AITLS中起致病作用。
Angioimmunoblastic T cell lymphoma (AITL) is probably the most common peripheral T-cell lymphoma. This pathology, although rare, is more common in Europe than in other regions. This lymphoma has a poor prognosis. AITL is very commonly associated with the Epstein-Barr virus (EBV) although the virus is not often found in neoplastic T cells but rather in adjacent B cells. Our objective was to study the transcriptome of EBV in AITLs comparatively to other EBV-associated lymphomas and to compare the results with those obtained for cell lines. We showed in AITLs a strong expression of Bam-HI A rightward transcripts (BARTs) more expressed than in the other lymphomas and especially than in cell lines. BARTs can participate in tumor development. We also showed a latency IIc in AITLs with the expression of BNLF2a and BCRF1 genes which may participate in the survival of infected cells. These results support the involvement of EBV in AITLs. The Epstein–Barr virus (EBV) is associated with angioimmunoblastic T cell lymphoma (AITL) in more than 80% of cases. Few studies have focused on this association and it is not clear now what role the virus plays in this pathology. We used next-generation sequencing (NGS) to study EBV transcriptome in 14 AITLs compared to 21 other lymphoma samples and 11 cell lines including 4 lymphoblastoid cell lines (LCLs). Viral transcripts were recovered using capture probes and sequencing was performed on Illumina. Bam-HI A rightward transcripts (BARTs) were the most latency transcripts expressed in AITLs, suggesting they may play a role in this pathology. Thus, BARTs, already described as highly expressed in carcinoma cells, are also very present in AITLs and other lymphomas. They were poorly expressed in cell lines other than LCLs. AITLs showed a latency IIc, with BNLF2a gene expression. For most AITLs, BCRF1, which encodes a homologous protein of human interleukin 10, vIL-10, was in addition expressed. This co-expression can contribute to immune escape and survival of infected cells. Considering these results, it can be assumed that EBV plays a pathogenic role in AITLs.
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影响因子：
9.8
作者：
Altmann, Markus;Hammerschmidt, Wolfgang
通讯作者：
Hammerschmidt, Wolfgang
影响因子：
5.4
作者：
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影响因子：
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通讯作者：
Wiels J
影响因子：
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作者：
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通讯作者：
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