Zbtb16 mediates a switch between Fgf signalling regimes in the developing hindbrain.
Zbtb16 mediates a switch between Fgf signalling regimes in the developing hindbrain.
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DOI：
10.1242/dev.201319
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发表时间：
2023-09-15
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影响因子：
--
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细胞外信号在特定的时间开启和关闭，使发育中的组织顺序形成图案。在斑马鱼的后脑，成纤维细胞生长因子（Fgf）信号在不同的发育阶段有不同的作用：在早期的后脑，短暂的Fgf3和Fgf8信号从菱形4是正确的分割所需的，而后来，神经元Fgf20的表达限制神经发生到特定的空间域内的每个菱形。这两种信号机制之间的转换如何协调尚不清楚。我们目前的证据表明，Zbtb16转录因子是需要这种转变发生在一个有序的方式。Zbtb16在早期的前脑中表达较高，然后逐渐上调，并局限于神经祖细胞。在缺乏功能性Zbtb16的突变体中，fgf3表达未能下调并持续到晚期，导致神经发生期间过量和更广泛的FGF信号传导。因此，神经发生的空间模式在Zbtb16突变体中被破坏。我们的研究结果揭示了不同阶段的特定作用的FGF信号是如何协调在斑马鱼后脑。总结：Zbtb16的动态表达是斑马鱼后脑早期节段性FGF3表达下调的基础，使FGF20组织神经发生的后期阶段的FGF20信号传导模式正确。
Developing tissues are sequentially patterned by extracellular signals that are turned on and off at specific times. In the zebrafish hindbrain, fibroblast growth factor (Fgf) signalling has different roles at different developmental stages: in the early hindbrain, transient Fgf3 and Fgf8 signalling from rhombomere 4 is required for correct segmentation, whereas later, neuronal Fgf20 expression confines neurogenesis to specific spatial domains within each rhombomere. How the switch between these two signalling regimes is coordinated is not known. We present evidence that the Zbtb16 transcription factor is required for this transition to happen in an orderly fashion. Zbtb16 expression is high in the early anterior hindbrain, then gradually upregulated posteriorly and confined to neural progenitors. In mutants lacking functional Zbtb16, fgf3 expression fails to be downregulated and persists until a late stage, resulting in excess and more widespread Fgf signalling during neurogenesis. Accordingly, the spatial pattern of neurogenesis is disrupted in Zbtb16 mutants. Our results reveal how the distinct stage-specific roles of Fgf signalling are coordinated in the zebrafish hindbrain. Summary: Dynamic expression of Zbtb16 underlies downregulation of early segmental fgf3 expression in the zebrafish hindbrain, enabling the correct pattern of Fgf signalling at later stages when Fgf20 organizes neurogenesis.
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影响因子：
9.8
作者：
Gaber ZB;Butler SJ;Novitch BG
通讯作者：
Novitch BG
影响因子：
4.6
作者：
Breau, Marie A.;Wilson, Duncan;Xu, Qiling
通讯作者：
Xu, Qiling
影响因子：
4.6
作者：
Hammond, Katherine L.;Whitfield, Tanya T.
通讯作者：
Whitfield, Tanya T.
DOI：
10.1073/pnas.92.6.2249
发表时间：
1995-03-14
影响因子：
11.1
作者：
COOK, M;GOULD, A;ZELENT, A
通讯作者：
ZELENT, A
影响因子：
2.5
作者：
HEYMAN, I;FAISSNER, A;LUMSDEN, A
通讯作者：
LUMSDEN, A