Association of alanine-valine manganese superoxide dismutase gene polymorphism and microheterogeneity manganese superoxide dismutase activity in breast cancer and benign breast tissue.
Association of alanine-valine manganese superoxide dismutase gene polymorphism and microheterogeneity manganese superoxide dismutase activity in breast cancer and benign breast tissue.
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DOI：
10.4048/jbc.2012.15.2.157
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发表时间：
2012-06
影响因子：
2.4
通讯作者：
Abdeldayem SA
中科院分区：
文献类型：
--
作者：
Atoum M;Abdel-Fattah M;Nimer N;Abdel-Rahman S;Abdeldayem SA
虽然乳腺癌的病因是多因素的，但氧化应激在癌发生中起重要作用。在这项研究中，锰超氧化物歧化酶（MnSOD）基因多态性和活性在良性和乳腺癌组织进行了评估。101名女性入组本研究，其中65名经组织病理学诊断为乳腺癌，46名为良性患者。MnSOD酶活性测定采用间接竞争抑制法，MnSOD基因多态性测定采用聚合酶链反应和琼脂糖凝胶电泳。乳腺癌组织中MnSOD酶活性（79.83±42.14）低于良性肿瘤组织（236.18±46.37）。Ala/瓦尔患者乳腺癌组织中MnSOD酶活性（39.19±7.33）明显低于瓦尔/瓦尔患者乳腺癌组织中MnSOD酶活性（96.9±22.9）。瓦尔/瓦尔癌组织中MnSOD酶活性（96.9±22.9）明显低于良性组织（255.44±42.7）。乳腺癌肿瘤含有较少的MnSOD比良性乳腺样品。与瓦尔/瓦尔乳腺癌患者相比，Ala/瓦尔多态性患者的MnSOD活性降低。Ala/瓦尔基因多态性可能是乳腺癌分期越晚的危险因素。MnSOD基因Ala/瓦尔多态性可能是乳腺癌发生发展的危险因素，MnSOD活性降低可能是乳腺癌发生发展的机制之一。需要进一步的研究来评估MnSOD在乳腺癌进展中的作用。
Although the etiology of breast cancer is multifactorial, oxidative stress plays an important role in carcinogenesis. In this study, manganese superoxide dismutase (MnSOD) gene polymorphism and activity were evaluated in benign and breast cancer tissue. One hundred and one females were enrolled in this study, 65 who were histopathologically diagnosed with breast cancer and 46 who were benign patients. MnSOD enzyme activity was determined using an indirect competitive inhibition assay and MnSOD gene polymorphism using poly merase chain reaction and agarose gel electrophoresis. MnSOD enzymatic activity (79.83±42.14) was lower in breast cancer tissue compared to benign tumors (236.18±46.37). At the same time, MnSOD enzymatic activity among Ala/Val patients was significantly lower in breast cancer tissue (39.19±7.33) than in Val/Val malignant breast tumors tissue (96.9±22.9). MnSOD enzymatic activity was significantly lower in Val/Val cancer tissue (96.9±22.9) than in benign tissue (255.44±42.7). Breast cancer tumors contain less MnSOD than benign breast samples. Patients with Ala/Val polymorphism had reduced MnSOD activity compared to patients with Val/Val breast cancer. Ala/Val gene polymorphism may be a risk factor associated with more advanced breast cancer stage. MnSOD gene polymorphism Ala/Val may be a risk factor associated with more advanced breast cancer stage, and reduction of MnSOD activity may be a mechanism of the progression of benign to malignant tumors. Further investigations are needed to evaluate the role of MnSOD in breast cancer progression.
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影响因子：
3.5
作者：
Holley, Aaron K.;Dhar, Sanjit Kumar;Xu, Yong;St Clair, Daret K.
通讯作者：
St Clair, Daret K.
DOI：
10.1186/bcr1628
发表时间：
2007
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Gago-Dominguez M;Jiang X;Castelao JE
通讯作者：
Castelao JE
影响因子：
--
作者：
Mukhopadhyay, S;Das, SK;Mukherjee, S
通讯作者：
Mukherjee, S
DOI：
10.1097/00008571-200303000-00004
发表时间：
2003-03-01
期刊：
PHARMACOGENETICS
影响因子：
--
作者：
Sutton, A;Khoury, H;Degoul, F
通讯作者：
Degoul, F
DOI：
10.1073/pnas.93.9.4471
发表时间：
1996-04-30
影响因子：
11.1
作者：
Rosenblum, JS;Gilula, NB;Lerner, RA
通讯作者：
Lerner, RA