Novel rodent models for macular research.
Novel rodent models for macular research.
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DOI：
10.1371/journal.pone.0013403
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发表时间：
2010-10-15
期刊：
影响因子：
3.7
通讯作者：
Beck SC
中科院分区：
文献类型：
--
作者：
Huber G;Heynen S;Imsand C;vom Hagen F;Muehlfriedel R;Tanimoto N;Feng Y;Hammes HP;Grimm C;Peichl L;Seeliger MW;Beck SC
许多致残的人类视网膜疾病涉及中央视网膜，特别是黄斑。然而，研究中常用的啮齿动物模型，小鼠和大鼠，不具有黄斑。本研究的目的是确定一个显着的中央区域的小型实验室啮齿动物作为黄斑研究的潜在新模型。 使用适用于小啮齿动物眼睛的标准设备（Heidelberg Engineering HRA 1和Spectralis™），对视网膜研究中不常用的实验室啮齿动物苍白沙鼠、长爪沙鼠和坎贝足鼠进行共焦扫描激光检眼镜检查（cSLO）、荧光素和吲哚菁绿色血管造影术以及光谱域光学相干断层扫描（SD-OCT）。根据血管地形图、视网膜厚度、视网膜神经节细胞和视锥光感受器的地形图评估视条纹样图案的存在。所有三个物种的研究表明，一个显着的水平条纹状专业化的证据。cSLO血管造影术和视网膜整体计数显示，表层视网膜血管通常在位于视神经背侧的相应区域的边界处分叉并变窄成稀疏的毛细血管网。与黄斑区相似，条纹区没有较大的血管。此外，在视纹区，感光细胞层的厚度和神经节细胞层中神经元的群体密度显著增加。沙鼠perpallidus，长爪沙鼠和Photopus campbelli的视网膜特化类似于灵长类动物黄斑的功能。因此，本文报道的啮齿动物可用于研究黄斑发育和疾病（如年龄相关性黄斑变性和糖尿病性黄斑水肿）的各个方面，以及治疗策略的临床前评估。
Many disabling human retinal disorders involve the central retina, particularly the macula. However, the commonly used rodent models in research, mouse and rat, do not possess a macula. The purpose of this study was to identify small laboratory rodents with a significant central region as potential new models for macular research. Gerbillus perpallidus, Meriones unguiculatus and Phodopus campbelli, laboratory rodents less commonly used in retinal research, were subjected to confocal scanning laser ophthalmoscopy (cSLO), fluorescein and indocyanine green angiography, and spectral-domain optical coherence tomography (SD-OCT) using standard equipment (Heidelberg Engineering HRA1 and Spectralis™) adapted to small rodent eyes. The existence of a visual streak-like pattern was assessed on the basis of vascular topography, retinal thickness, and the topography of retinal ganglion cells and cone photoreceptors. All three species examined showed evidence of a significant horizontal streak-like specialization. cSLO angiography and retinal wholemounts revealed that superficial retinal blood vessels typically ramify and narrow into a sparse capillary net at the border of the respective area located dorsal to the optic nerve. Similar to the macular region, there was an absence of larger blood vessels in the streak region. Furthermore, the thickness of the photoreceptor layer and the population density of neurons in the ganglion cell layer were markedly increased in the visual streak region. The retinal specializations of Gerbillus perpallidus, Meriones unguiculatus and Phodopus campbelli resemble features of the primate macula. Hence, the rodents reported here may serve to study aspects of macular development and diseases like age-related macular degeneration and diabetic macular edema, and the preclinical assessment of therapeutic strategies.
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影响因子：
4.4
作者：
Huber G;Beck SC;Grimm C;Sahaboglu-Tekgoz A;Paquet-Durand F;Wenzel A;Humphries P;Redmond TM;Seeliger MW;Fischer MD
通讯作者：
Fischer MD
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120.7
作者：
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通讯作者：
Lietman, T
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作者：
Bobu, Corina;Lahmam, Mohamed;Hicks, David
通讯作者：
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