Type 1 diabetes predisposes to enhanced gingival leukocyte margination and macromolecule extravasation in vivo.
Type 1 diabetes predisposes to enhanced gingival leukocyte margination and macromolecule extravasation in vivo.
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DOI：
10.1111/j.1600-0765.2010.01295.x
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发表时间：
2010-12
影响因子：
3.5
通讯作者：
Gyurko R
中科院分区：
文献类型：
--
作者：
Sima C;Rhourida K;Van Dyke TE;Gyurko R
糖尿病易患牙周病。然而，连接这两种情况的细胞和分子机制尚不清楚。在体内观察慢性高血糖对牙龈血管白细胞边缘和大分子外渗的影响。采用牙龈活体显微镜观察糖尿病秋田小鼠和健康野生型小鼠的异硫氰酸荧光素-葡聚糖外渗情况。注射罗丹明6G和FITC-LY6G分别用于非特异性和多态核特异性白细胞标记。流式细胞术和western blotting检测细胞表面白细胞粘附分子的表达。与WT相比，秋田牙龈血管通透性明显增加[通透性指数（PI）： WT, 0.75±0.05；秋田，1.1±0.03:p < 0.05)。野生型牙龈血管在龈内注射肿瘤坏死因子α (TNFα) 2 h后通透性达到相当水平，作为阳性对照（PI, 1.17±0.16）。糖尿病牙龈滚动白细胞数量显著升高（WT, 25±3.7 cells/min；秋田，42±8.5 cells/min; p < 0.03）。灌胃TNFα后，WT细胞的滚动细胞计数相似（10 ng TNFα, 47±1.3个细胞/min； 100 ng TNFα, 57.5±5.85个细胞/min）。在WT小鼠和秋田小鼠中，紧紧附着在内皮上的白细胞数量相似。与WT相比，秋田小鼠白细胞表面p -选择素糖蛋白配体1和CD11a的表达增加，而l-选择素的表达不变。此外，与WT相比，秋田小鼠牙龈组织中p -选择素的表达升高。慢性高血糖诱导牙龈微循环的促炎状态，其特征是血管通透性增加，白细胞和内皮细胞活化。白细胞诱导的微血管损伤反过来又可能导致糖尿病患者牙周组织损伤。
Diabetes predisposes to periodontal disease. However, the cellular and molecular mechanisms linking the two conditions are not clear. The impact of chronic hyperglycemia on leukocyte margination and macromolecule extravasation was determined in gingival vessels in vivo. Gingival intravital microscopy was employed to measure extravasation of fluorescein isothiocyanate (FITC)–dextran in diabetic Akita and healthy wild-type (WT) mice. Rhodamine 6G and FITC–LY6G were injected for nonspecific and polymorphonuclear-specific leukocyte labeling, respectively. Surface expression of leukocyte adhesion molecules was determined with flow cytometry and western blotting. Vascular permeability was significantly increased in Akita gingival vessels compared with WT [permeability index (PI): WT, 0.75 ± 0.05; Akita, 1.1 ± 0.03: p < 0.05). Wild-type gingival vessels reached comparable permeability 2 h after intragingival injection of tumor necrosis factor α (TNFα), used here as positive control (PI, 1.17 ± 0.16). The number of rolling leukocytes was significantly elevated in diabetic gingiva (WT, 25 ± 3.7 cells/min; Akita, 42 ± 8.5 cells/min; p < 0.03). Similar rolling cell counts were obtained in WT after intragingival injection of TNFα (10 ng TNFα, 47 ± 1.3 cells/min; 100 ng TNFα, 57.5 ± 5.85 cells/min). The number of leukocytes firmly attached to the endothelium was similar in WT and Akita mice. Leukocyte cell-surface expression of P-selectin glycoprotein ligand-1 and CD11a was increased in Akita mice, while L-selectin remained unchanged when compared with WT. Moreover, P-selectin expression in Akita gingival tissues was elevated compared with that of WT. Chronic hyperglycemia induces a proinflammatory state in the gingival microcirculation characterized by increased vascular permeability, and leukocyte and endothelial cell activation. Leukocyte-induced microvascular damage, in turn, may contribute to periodontal tissue damage in diabetes.
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影响因子：
3.1
作者：
GOLUB, LM;NICOLL, GA;RAMAMURTHY, NS
通讯作者：
RAMAMURTHY, NS
DOI：
10.1080/10623320500192016
发表时间：
2005-05-01
影响因子：
--
作者：
Hwa, C;Sebastian, A;Aird, WC
通讯作者：
Aird, WC
影响因子：
4.4
作者：
Gyurko, Robert;Siqueira, Camille C.;Van Dyke, Thomas E.
通讯作者：
Van Dyke, Thomas E.
影响因子：
2.6
作者：
Asaduzzaman, Muhammad;Mihaescu, Andrada;Thorlacius, Henrik
通讯作者：
Thorlacius, Henrik
影响因子：
6.7
作者：
Gapski, Ricardo;Hasturk, Hatice;Giannobile, William V.
通讯作者：
Giannobile, William V.