Localisation and interactions of the Vipp1 protein in cyanobacteria.
Localisation and interactions of the Vipp1 protein in cyanobacteria.
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DOI：
10.1111/mmi.12826
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发表时间：
2014-10-13
影响因子：
3.6
通讯作者：
Mullineaux CW
中科院分区：
文献类型：
--
作者：
Bryan SJ;Burroughs NJ;Shevela D;Yu J;Rupprecht E;Liu LN;Mastroianni G;Xue Q;Llorente-Garcia I;Leake MC;Eichacker LA;Schneider D;Nixon PJ;Mullineaux CW
Vipp 1蛋白在蓝细菌和叶绿体中对于维持光合功能和类囊体膜结构至关重要。为了研究其作用模式，我们产生了蓝细菌集胞藻属PCC 6803和集胞藻属PCC 7942的菌株，其中Vipp 1在C-末端用绿色荧光蛋白标记，并从天然染色体位点表达。功能几乎没有受到干扰。活细胞荧光成像显示Vipp 1在强光下发生了戏剧性的重新定位。在弱光下，Vipp 1主要分散在细胞质中，偶尔集中在类囊体膜的外周。强光诱导Vipp 1聚结成本地化的斑点在几分钟内，与净搬迁Vipp 1的细胞质膜和类囊体膜附近。下拉和质谱鉴定了大量的蛋白质，这些蛋白质仅在强光照射后与Vipp 1直接或间接相关。这些不仅包括光合和胁迫相关蛋白，还包括RNA加工，翻译和蛋白质组装因子。这表明Vipp 1点可能参与蛋白质组装。一种可能性是Vipp 1参与了应激诱导的局部蛋白质组装中心的形成，从而在应激条件下增强了蛋白质合成并将其递送至膜。
The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green fluorescent protein at the C-terminus and expressed from the native chromosomal locus. There was little perturbation of function. Live-cell fluorescence imaging shows dramatic relocalisation of Vipp1 under high light. Under low light, Vipp1 is predominantly dispersed in the cytoplasm with occasional concentrations at the outer periphery of the thylakoid membranes. High light induces Vipp1 coalescence into localised puncta within minutes, with net relocation of Vipp1 to the vicinity of the cytoplasmic membrane and the thylakoid membranes. Pull-downs and mass spectrometry identify an extensive collection of proteins that are directly or indirectly associated with Vipp1 only after high-light exposure. These include not only photosynthetic and stress-related proteins but also RNA-processing, translation and protein assembly factors. This suggests that the Vipp1 puncta could be involved in protein assembly. One possibility is that Vipp1 is involved in the formation of stress-induced localised protein assembly centres, enabling enhanced protein synthesis and delivery to membranes under stress conditions.
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影响因子：
7.4
作者：
Barthel, Sandra;Bernat, Gabor;Schneider, Dirk
通讯作者：
Schneider, Dirk
影响因子：
3.2
作者：
Esther Perez-Perez, Maria;Mata-Cabana, Alejandro;Florencio, Francisco J.
通讯作者：
Florencio, Francisco J.
叶绿体靶向的 Hsp90 在拟南芥质体发育和胚胎发生中发挥重要作用，可能与 VIPP1 相关。
影响因子：
6.4
作者：
Feng, Juanjuan;Fan, Pengxiang;Li, Yinxin
通讯作者：
Li, Yinxin
影响因子：
4.8
作者：
Kojima, Kouji;Motohashi, Ken;Nishiyama, Yoshitaka
通讯作者：
Nishiyama, Yoshitaka
影响因子：
2.9
作者：
Liberton, Michelle;Berg, R. Howard;Pakrasi, Himadri B.
通讯作者：
Pakrasi, Himadri B.