MiR-503 promotes wound healing of diabetic foot ulcer by targeting FBN1
MiR-503 promotes wound healing of diabetic foot ulcer by targeting FBN1
复制标题
MiR-503 通过靶向 FBN1 促进糖尿病足溃疡伤口愈合
DOI：
10.4103/1995-7645.228441
复制
发表时间：
2018-03
影响因子：
3.1
通讯作者：
Bi Chang Long
中科院分区：
文献类型：
--
作者：
Wang Ming Li;Chen Jing;Zhou Yue;Zhao Yu Jie;Sun De Rong;Wu Qiang;Bi Chang Long
目的：探讨miR-503与糖尿病足溃疡创面愈合的关系。方法：采用基因芯片技术检测DFU组织与正常组织之间的miRNAs表达。采用定量逆转录聚合酶链式反应技术检测miR-503在DFU患者组织和血清中的表达。CCK-8比色法检测细胞增殖情况。用原位末端标记法检测miR-503作用后细胞的凋亡率。根据RNA22网站上的数据预测miR-503与fbillin1(FBN1)之间可能的相关性，并通过荧光素酶报告基因分析进行验证。结果：基因芯片分析显示，与正常组织相比，DFU组织中miR-503的表达水平显著降低。定量逆转录聚合酶链式反应证实，DFU患者组织和血清中miR-503的表达明显增加。CCK-8检测结果表明，miR-503模拟物的转染明显促进了细胞的增殖。然而，TUNEL检测表明miR-503模拟物通过抑制细胞的凋亡来提高成纤维细胞的存活率。此外，miR-503AMO在DFU组织成纤维细胞中的作用与miR-503模拟治疗完全相反。RNA22预测，MIR-503是对FBN1的补充。此外，siRNA-FBN1促进成纤维细胞的增殖，但抑制成纤维细胞的凋亡。结论：MIR-503通过直接靶向FBN1调控DFU患者成纤维细胞功能和创面愈合，为DFU的诊断和治疗提供了新的关键靶点。
Objective: To highlight the relationship between miR-503 and wound healing of diabetic foot ulcer (DFU). Methods: Microarray analysis was used to detect the dysregulated miRNAs between the DFU tissues and normal tissues. The expression of miR-503 in tissues and serum of patients with DFU was detected by qRT-PCR technique. Then, CCK-8 assay was applied to determine the cell proliferation. TUNEL assay was used for assessing the apoptosis of cells after treatment with miR-503. Possible correlation between miR-503 and fbillin1 (FBN1) was predicted according to data accessed on RNA22 website online, and was detected for confirmation by luciferase reporter assay. Results: Microarray analysis showed that miR- 503 was significantly decreased in the DFU tissues compared with normal tissues. While marked increase in the expression of miR-503 in tissues and serum of patients with DFU was confirmed by qRT-PCR technique. Then, CCK-8 assay indicated that transfection of miR- 503 mimic obviously accelerated the cell proliferation. However, TUNEL assays suggested that miR-503 mimic inhibited the apoptosis of cells to improve the survival of fibroblasts. Besides, miR-503 AMO played a role in fibroblasts of DFU tissues exactly countering to miR-503 mimic treatment. It was predicted that MiR-503 is a complementary to the FBN1 by RNA22. Besides, SiRNA-FBN1 promoted the proliferation, but brought down the apoptosis of fibroblasts. Conclusions: MiR-503 regulates the function of fibroblasts and wound healing of patients with DFU by targeting FBN1 directly which provids a novel and critical target for diagnosis and treatment of DFU.
登录
查看更多内容
基因型 - 表型相关性在MARFAN综合征的临床管理中的重要性。
影响因子：
3.7
作者：
Becerra-Muñoz VM;Gómez-Doblas JJ;Porras-Martín C;Such-Martínez M;Crespo-Leiro MG;Barriales-Villa R;de Teresa-Galván E;Jiménez-Navarro M;Cabrera-Bueno F
通讯作者：
Cabrera-Bueno F
影响因子：
2.9
作者：
Nelson A;Wright-Hughes A;Backhouse MR;Lipsky BA;Nixon J;Bhogal MS;Reynolds C;Brown S;CODIFI collaborators
通讯作者：
CODIFI collaborators
影响因子：
3.7
作者：
Saxena T;Tandon B;Sharma S;Chameettachal S;Ray P;Ray AR;Kulshreshtha R
通讯作者：
Kulshreshtha R
DOI：
10.1016/j.bbrc.2017.09.099
发表时间：
2017-11-18
影响因子：
3.1
作者：
Li, Zhao-yun;Zhu, Shan-shan;Zhang, Li-ming
通讯作者：
Zhang, Li-ming
影响因子：
--
作者：
D. Satterfield;L. Debruyn;Marjorie Santos;L. Alonso;Melinda Frank
通讯作者：
D. Satterfield;L. Debruyn;Marjorie Santos;L. Alonso;Melinda Frank