Identification of the neuropeptide transmitter proctolin in Drosophila larvae: characterization of muscle fiber-specific neuromuscular endings
Identification of the neuropeptide transmitter proctolin in Drosophila larvae: characterization of muscle fiber-specific neuromuscular endings
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果蝇幼虫中神经肽递质 proctolin 的鉴定：肌纤维特异性神经肌肉末梢的表征
DOI：
10.1523/jneurosci.08-01-00242.1988
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发表时间：
1988
影响因子：
3.9
通讯作者：
H. Keshishian
中科院分区：
文献类型：
--
作者：
MaryDilys S. Anderson;M. Halpern;H. Keshishian
研究了黑腹果蝇（Drosophila melanogaster）幼虫中肽神经递质proctolin的细胞定位。利用反相高效液相色谱法从中枢神经系统、后肠和节段体壁中回收Proctolin，并通过生物测定、免疫测定和酶分析对其进行了表征。在幼虫的中枢神经系统中发现了一个小的、定型的proctolin免疫反应神经元群。一些被识别的神经元可能是传出神经。在外周，在内脏和骨骼肌纤维上发现了proctolin免疫反应性神经肌肉末梢。在后肠，神经肽与内在圆形肌纤维的末梢有关。我们提出后肠肌纤维是由类似于先前描述的蚱蜢的顺肽能传出神经的中枢神经元支配的。节段性身壁神经支配由若干可单独识别的肌纤维上的节段特异性连接模式组成。虽然人们普遍认为果蝇肌纤维是由谷氨酸运动神经元支配的，但我们的数据表明，肌肉纤维的一个特殊子集也受肽能神经支配。
The cellular localization of the peptide neurotransmitter proctolin was determined for larvae of the fruitfly Drosophila melanogaster. Proctolin was recovered from the CNS, hindgut, and segmental bodywall using reverse-phase HPLC, and characterized by bioassay, immunoassay, and enzymatic analysis. A small, stereotyped population of proctolin- immunoreactive neurons was found in the larval CNS. Several of the identified neurons may be efferents. In the periphery, proctolin- immunoreactive neuromuscular endings were identified on both visceral and skeletal muscle fibers. On the hindgut, the neuropeptide is associated with endings on intrinsic circular muscle fibers. We propose that the hindgut muscle fibers are innervated by central neurons homologous to previously described proctolinergic efferents of grasshoppers. The segmental bodywall innervation consists of a pattern of segment-specific junctions on several singly identifiable muscle fibers. While it is generally accepted that Drosophila muscle fibers are innervated by glutamatergic motoneurons, our data indicate that a specialized subset of muscle fibers are also innervated by peptidergic efferents.
神经肽 proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH)：昆虫中枢神经系统的免疫检测和神经元定位。
DOI：
10.1073/pnas.78.9.5899
发表时间：
1981
影响因子：
11.1
作者：
Bishop,CA;O'Shea,M;Miller,RJ
通讯作者：
Miller,RJ