Tracking Eye Movements During Sleep in Mice.
Tracking Eye Movements During Sleep in Mice.
复制标题
追踪小鼠睡眠期间的眼球运动
DOI：
10.3389/fnins.2021.616760
复制
发表时间：
2021
影响因子：
4.3
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Meng Q;Tan X;Jiang C;Xiong Y;Yan B;Zhang J
眼动不仅是为了调节视野，维持视网膜上视觉信息的稳定，也是大脑认知状态的一种外在表现。最近的研究表明，清醒和快速眼动（REM）睡眠之间的眼动模式相似，这表明快速眼动睡眠的大脑状态很可能与清醒状态相似。根据眼动频率的不同，人类快速眼动睡眠可分为相性快速眼动睡眠和强直性快速眼动睡眠。小鼠是研究睡眠的神经和分子机制最常用的动物模型。然而，由于缺乏快速眼动睡眠期间眼动模式的细节，因此小鼠快速眼动睡眠是否可以进一步划分为不同的阶段仍然是未知的。在此，我们开发了一种结合脑电图（EEG）、肌电图（EMG）和眼动记录的设备来研究小鼠睡眠时的眼动模式。我们在眼结膜下植入一块磁铁，用磁传感器跟踪眼球运动。在头固定的小鼠中，磁信号与视觉成像有很强的相关性，表明磁信号反映了眼球运动的方向和幅度。我们还发现植入结膜下的磁体具有良好的生物相容性。最后，我们对睡眠-觉醒周期的眼动进行了检测，并根据眼动频率区分了强张性快速眼动和相性快速眼动，发现与强张性快速眼动相比，相性快速眼动在0.50 Hz时振荡功率更高，在1.50 ~ 7.25 Hz和9.50 ~ 12.00 Hz时振荡功率更低。我们的设备可以同时记录睡眠和清醒时的脑电图、肌电图和眼动，为研究睡眠中的眼动和其他小鼠研究提供了方便和高时空分辨率的工具。
Eye movement is not only for adjusting the visual field and maintaining the stability of visual information on the retina, but also provides an external manifestation of the cognitive status of the brain. Recent studies showed similarity in eye movement patterns between wakefulness and rapid eye movement (REM) sleep, indicating that the brain status of REM sleep likely resembles that of awake status. REM sleep in humans could be divided into phasic REM and tonic REM sleep according to the difference in eye movement frequencies. Mice are the most commonly used animal model for studying neuronal and molecular mechanisms underlying sleep. However, there was a lack of details for eye movement patterns during REM sleep, hence it remains unknown whether REM sleep can be further divided into different stages in mice. Here we developed a device combining electroencephalogram (EEG), electromyogram (EMG) as well as eye movements recording in mice to study the eye movement patterns during sleep. We implanted a magnet beneath the conjunctiva of eye and tracked eye movements using a magnetic sensor. The magnetic signals showed strong correlation with video-oculography in head-fixed mice, indicating that the magnetic signals reflect the direction and magnitude of eye movement. We also found that the magnet implanted beneath the conjunctiva exhibited good biocompatibility. Finally, we examined eye movement in sleep–wake cycle, and discriminated tonic REM and phasic REM according to the frequency of eye movements, finding that compared to tonic REM, phasic REM exhibited higher oscillation power at 0.50 Hz, and lower oscillation power at 1.50–7.25 Hz and 9.50–12.00 Hz. Our device allowed to simultaneously record EEG, EMG, and eye movements during sleep and wakefulness, providing a convenient and high temporal-spatial resolution tool for studying eye movements in sleep and other researches in mice.
登录
查看更多内容
影响因子：
3
作者：
Kaneko, Chris R. S.;Rosenfeld, Sam;Fontaine, Ethan;Markov, Alex;Phillips, James O.;Yarno, John
通讯作者：
Yarno, John
影响因子：
4.4
作者：
Ermis, Ummehan;Krakow, Karsten;Voss, Ursula
通讯作者：
Voss, Ursula
非侵入性跟踪眼睛：同时比较猕猴中的巩膜搜索线圈和光学跟踪技术。
影响因子：
3
作者：
Kimmel DL;Mammo D;Newsome WT
通讯作者：
Newsome WT
影响因子：
4.8
作者：
Arnaldi, Dario;Latimier, Alice;Arnulf, Isabelle
通讯作者：
Arnulf, Isabelle
影响因子：
5.4
作者：
Jia, Yingxin;Tyler, Christopher W.
通讯作者：
Tyler, Christopher W.