Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns
Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns
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仓鼠附睾精子活力的成熟以及仓鼠和大鼠精子硫醇状态对其活力模式的影响
DOI：
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发表时间：
1994
影响因子：
2.5
通讯作者：
T. Cooper
中科院分区：
文献类型：
--
作者：
C. Yeung;G. Oberländer;T. Cooper
使用计算机系统建立了一种客观量化仓鼠精子运动参数作为附睾成熟指标的方法。对五个附睾区域释放到培养基中的精子的分析表明，运动百分比和曲线速度（VCL）最急剧的增加发生在从远端体到近端尾部的开始处，以及直线速度（VSL）从开始到近尾部区域内更远端的部位。高渗透压（400 mOsm/kg）和硫醇氧化剂二酰胺（10 μM）均增加了远端精子体鞭毛的直线度，但仅后者增加了 VSL。硫醇还原剂二硫苏糖醇（DTT，1mM）刺激并维持头部精子的运动百分比和速度，仅刺激远端精子的百分比运动，但在长时间孵育中降低了近尾精子的速度。在大鼠中，二酰胺增加了精子路径的直线度，但没有增加精子的速度，但在 15 分钟内导致精子不动，而 DTT 则延长了体外精子活力的维持时间。 DTT 存在下运动学参数的轻微增加通过二酰胺预孵育 2 分钟得到增强。 DTT 和二酰胺的作用不是代偿性的这一发现表明，SH/S-S 状态对精子运动的影响是多方面的，精子成熟时对刺激的敏感性降低。 © 1994 Wiley-Liss, Inc.
A method for objective quantification of hamster sperm movement parameters as an indicator of maturation along the epididymis was established using a computerised system. Analysis of spermatozoa released into medium from five epididymal regions showed that the most drastic increases in percentage motility and curvilinear velocity (VCL) occurred from the distal corpus to the beginning of the proximal cauda and in straight‐line velocity (VSL) from the beginning to a more distal site within the proximal cauda region. Both high osmolarity (400 mOsm/kg) and the thiol‐oxidising agent diamide (10 μM) increased flagellar straightness of distal corpus spermatozoa, but VSL was increased only with the latter. The thiol‐reducing agent dithiothreitol (DTT, 1mM) stimulated and maintained percentage motility and velocities of spermatozoa from the caput, stimulated only percentage motility of distal corpus sperm, but decreased velocities of those from the proximal cauda in prolonged incubation. In rats, diamide increased path straightness but not velocities of caput spermatozoa and yet caused immotility within 15 min, whereas DTT prolonged the maintenance of in vitro motility. The slight increases in kinematic parameters in the presence of DTT were enhanced by a 2‐min preincubation with diamide. The finding that the effects of DTT and diamide were not compensatory suggests that the influence of the SH/S‐S status on sperm movement is multifaceted, with decreasing sensitivity to stimulation upon sperm maturation. © 1994 Wiley‐Liss, Inc.
维拉帕米和巯基试剂对牛精子钙转运的影响。
影响因子：
3.6
作者：
Breitbart,H;Lardy,HA
通讯作者：
Lardy,HA
参与维持仓鼠精子鞭毛直线度的巯基蛋白的表征。
影响因子：
--
作者：
Cornwall,GA;Chang,TS
通讯作者：
Chang,TS
研究某些抗氧化剂在保持重新激活的公牛精子模型活力方面的有效性。
影响因子：
3.6
作者：
Lindemann,CB;O'Brien,JA;Giblin,FJ
通讯作者：
Giblin,FJ